DNA from keratinous tissue. Part II

DNA from keratinous tissue. Part II: feather

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

DNA from keratinous tissue. Part II : feather. / Olsen, Maia E.; Bengtsson, Camilla Friis; Bertelsen, Mads Frost; Willerslev, Eske; Gilbert, Tom.

I: Annals of Anatomy, Bind 194, Nr. 1, 2012, s. 31-35.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Olsen, ME, Bengtsson, CF, Bertelsen, MF, Willerslev, E & Gilbert, T 2012, 'DNA from keratinous tissue. Part II: feather', Annals of Anatomy, bind 194, nr. 1, s. 31-35. https://doi.org/10.1016/j.aanat.2011.03.003

APA

Olsen, M. E., Bengtsson, C. F., Bertelsen, M. F., Willerslev, E., & Gilbert, T. (2012). DNA from keratinous tissue. Part II: feather. Annals of Anatomy, 194(1), 31-35. https://doi.org/10.1016/j.aanat.2011.03.003

Vancouver

Olsen ME, Bengtsson CF, Bertelsen MF, Willerslev E, Gilbert T. DNA from keratinous tissue. Part II: feather. Annals of Anatomy. 2012;194(1):31-35. https://doi.org/10.1016/j.aanat.2011.03.003

Author

Olsen, Maia E. ; Bengtsson, Camilla Friis ; Bertelsen, Mads Frost ; Willerslev, Eske ; Gilbert, Tom. / DNA from keratinous tissue. Part II : feather. I: Annals of Anatomy. 2012 ; Bind 194, Nr. 1. s. 31-35.

Bibtex

@article{fb6fbcadc2a44f848e6175d89d9d996d,

title = "DNA from keratinous tissue. Part II: feather",

abstract = "Although good quality DNA can be recovered from the base of the calamus of freshly sampled feathers, as from other fully keratinized tissues such as nail or hair shaft, the quality and quantity of DNA in the majority of feather structures is much poorer. Little research has been performed to characterize the quality of this DNA is, and thus what a researcher might be able to achieve when using feathers as a source of DNA. In this review, we expand on our companion article detailing the quality of DNA in nail and hair, by synthesizing published, and new preliminary genetic data obtained from feathers. As with nail and hair, we demonstrate that although DNA can, in general, be recovered from all parts of the feather, the quality of such DNA varies. As such, although one can expect a priori that genetic analyses are possible on the feather, for PCR based analyses, it is extremely difficult to predict the size of amplicon that can be used in such analyses. However, PCR-free genetic analyses that can exploit much smaller DNA fragments may promise to be a powerful tool for future exploitation.",

author = "Olsen, {Maia E.} and Bengtsson, {Camilla Friis} and Bertelsen, {Mads Frost} and Eske Willerslev and Tom Gilbert",

year = "2012",

doi = "10.1016/j.aanat.2011.03.003",

language = "English",

volume = "194",

pages = "31--35",

journal = "Annals of Anatomy",

issn = "0940-9602",

publisher = "Elsevier GmbH",

number = "1",

}

RIS

TY - JOUR

T1 - DNA from keratinous tissue. Part II

T2 - feather

AU - Olsen, Maia E.

AU - Bengtsson, Camilla Friis

AU - Bertelsen, Mads Frost

AU - Willerslev, Eske

AU - Gilbert, Tom

PY - 2012

Y1 - 2012

N2 - Although good quality DNA can be recovered from the base of the calamus of freshly sampled feathers, as from other fully keratinized tissues such as nail or hair shaft, the quality and quantity of DNA in the majority of feather structures is much poorer. Little research has been performed to characterize the quality of this DNA is, and thus what a researcher might be able to achieve when using feathers as a source of DNA. In this review, we expand on our companion article detailing the quality of DNA in nail and hair, by synthesizing published, and new preliminary genetic data obtained from feathers. As with nail and hair, we demonstrate that although DNA can, in general, be recovered from all parts of the feather, the quality of such DNA varies. As such, although one can expect a priori that genetic analyses are possible on the feather, for PCR based analyses, it is extremely difficult to predict the size of amplicon that can be used in such analyses. However, PCR-free genetic analyses that can exploit much smaller DNA fragments may promise to be a powerful tool for future exploitation.

AB - Although good quality DNA can be recovered from the base of the calamus of freshly sampled feathers, as from other fully keratinized tissues such as nail or hair shaft, the quality and quantity of DNA in the majority of feather structures is much poorer. Little research has been performed to characterize the quality of this DNA is, and thus what a researcher might be able to achieve when using feathers as a source of DNA. In this review, we expand on our companion article detailing the quality of DNA in nail and hair, by synthesizing published, and new preliminary genetic data obtained from feathers. As with nail and hair, we demonstrate that although DNA can, in general, be recovered from all parts of the feather, the quality of such DNA varies. As such, although one can expect a priori that genetic analyses are possible on the feather, for PCR based analyses, it is extremely difficult to predict the size of amplicon that can be used in such analyses. However, PCR-free genetic analyses that can exploit much smaller DNA fragments may promise to be a powerful tool for future exploitation.

U2 - 10.1016/j.aanat.2011.03.003

DO - 10.1016/j.aanat.2011.03.003

M3 - Journal article

C2 - 21489767

VL - 194

SP - 31

EP - 35

JO - Annals of Anatomy

JF - Annals of Anatomy

SN - 0940-9602

IS - 1

ER -

ID: 34347810