Direct implementation of intestinal permeability test in nmr metabolomics for simultaneous biomarker discovery: a feasibility study in a preterm piglet model
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Direct implementation of intestinal permeability test in nmr metabolomics for simultaneous biomarker discovery : a feasibility study in a preterm piglet model. / Alinaghi, Masoumeh; Nguyen, Duc Ninh; Bertram, Hanne Christine; Sangild, Per Torp.
I: Metabolites, Bind 10, Nr. 1, 22, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Direct implementation of intestinal permeability test in nmr metabolomics for simultaneous biomarker discovery
T2 - a feasibility study in a preterm piglet model
AU - Alinaghi, Masoumeh
AU - Nguyen, Duc Ninh
AU - Bertram, Hanne Christine
AU - Sangild, Per Torp
PY - 2020
Y1 - 2020
N2 - Measurement of intestinal permeability (IP) is often used in the examination of inflammatory gastrointestinal disorders. IP can be assessed by measurement of urinary recovery of ingested non-metabolizable lactulose (L) and mannitol (M). The present study aimed to examine how measurements of IP can be integrated in a NMR-based metabolomics approach for a simultaneous quantification of L/M ratio and biomarker exploration. For this purpose, plasma and urine samples were collected from five-day-old preterm piglets (n = 20) with gastrointestinal disorders (subjected to intra-amniotic lipopolysaccharide (LPS, 1 mg/fetus)) after they had been administrated a 5% lactulose and 5% mannitol solution (15 mL/kg). The collected plasma and urine samples were analyzed by1H NMR-based metabolomics. Urine L/M ratio measured by1H NMR spectroscopy showed high correlation with the standard measurement of the urinary recoveries by enzymatic assays (r = 0.93, p < 0.05). Partial least squares (PLS) regressions and correlation analyses between L/M ratio and NMR metabolomics data revealed that L/M ratio was positively correlated with plasma lactate, acetate and succinate levels and negatively correlated with urinary hippuric acid and glycine. In conclusion, the present study demonstrated that NMR metabolomics enables simultaneous IP testing and discovery of biomarkers associated with an impaired intestinal permeability.
AB - Measurement of intestinal permeability (IP) is often used in the examination of inflammatory gastrointestinal disorders. IP can be assessed by measurement of urinary recovery of ingested non-metabolizable lactulose (L) and mannitol (M). The present study aimed to examine how measurements of IP can be integrated in a NMR-based metabolomics approach for a simultaneous quantification of L/M ratio and biomarker exploration. For this purpose, plasma and urine samples were collected from five-day-old preterm piglets (n = 20) with gastrointestinal disorders (subjected to intra-amniotic lipopolysaccharide (LPS, 1 mg/fetus)) after they had been administrated a 5% lactulose and 5% mannitol solution (15 mL/kg). The collected plasma and urine samples were analyzed by1H NMR-based metabolomics. Urine L/M ratio measured by1H NMR spectroscopy showed high correlation with the standard measurement of the urinary recoveries by enzymatic assays (r = 0.93, p < 0.05). Partial least squares (PLS) regressions and correlation analyses between L/M ratio and NMR metabolomics data revealed that L/M ratio was positively correlated with plasma lactate, acetate and succinate levels and negatively correlated with urinary hippuric acid and glycine. In conclusion, the present study demonstrated that NMR metabolomics enables simultaneous IP testing and discovery of biomarkers associated with an impaired intestinal permeability.
KW - Intestinal barrier dysfunction
KW - Lactulose
KW - Leaky gut
KW - Lipopolysaccharide (LPS)
KW - NMR metabolomics
KW - Prenatal inflammation
KW - Preterm infants
U2 - 10.3390/metabo10010022
DO - 10.3390/metabo10010022
M3 - Journal article
C2 - 31906404
AN - SCOPUS:85077847029
VL - 10
JO - Metabolites
JF - Metabolites
SN - 2218-1989
IS - 1
M1 - 22
ER -
ID: 236606117