Diet-dependent changes in the intestinal DNA methylome after introduction of enteral feeding in preterm pigs
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Diet-dependent changes in the intestinal DNA methylome after introduction of enteral feeding in preterm pigs. / Pan, Xiaoyu; Gong, Desheng; Gao, Fei; Sangild, Per Torp.
I: Epigenomics, Bind 10, Nr. 4, 01.04.2018, s. 395-408.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Diet-dependent changes in the intestinal DNA methylome after introduction of enteral feeding in preterm pigs
AU - Pan, Xiaoyu
AU - Gong, Desheng
AU - Gao, Fei
AU - Sangild, Per Torp
PY - 2018/4/1
Y1 - 2018/4/1
N2 - AIM: To examine how enteral feeding affects the intestinal epigenome and gene expression just after preterm birth.MATERIALS & METHODS: Intestinal tissue from preterm pigs, modeling preterm infants, was collected at birth and 5 days after gradual introduction of infant formula or bovine colostrum. The intestinal tissue was analyzed by reduced representation bisulfite sequencing and real-time qPCR.RESULTS: Relative to colostrum, formula increased bacterial epithelial adherence and lipopolysaccharide binding protein (LBP) expression, which was regulated by promoter methylation. Diet-dependent changes in DNA methylation and/or mRNA expression were related to innate immune response, hypoxia, angiogenesis and epithelial-mesenchymal transition pathways (e.g., TTC38, IL8, C3, HIF1A and VEGFR1).CONCLUSION: Epigenetic changes may mediate important effects of the first feeding on intestinal development in preterm neonates.
AB - AIM: To examine how enteral feeding affects the intestinal epigenome and gene expression just after preterm birth.MATERIALS & METHODS: Intestinal tissue from preterm pigs, modeling preterm infants, was collected at birth and 5 days after gradual introduction of infant formula or bovine colostrum. The intestinal tissue was analyzed by reduced representation bisulfite sequencing and real-time qPCR.RESULTS: Relative to colostrum, formula increased bacterial epithelial adherence and lipopolysaccharide binding protein (LBP) expression, which was regulated by promoter methylation. Diet-dependent changes in DNA methylation and/or mRNA expression were related to innate immune response, hypoxia, angiogenesis and epithelial-mesenchymal transition pathways (e.g., TTC38, IL8, C3, HIF1A and VEGFR1).CONCLUSION: Epigenetic changes may mediate important effects of the first feeding on intestinal development in preterm neonates.
U2 - 10.2217/epi-2017-0122
DO - 10.2217/epi-2017-0122
M3 - Journal article
C2 - 29587528
VL - 10
SP - 395
EP - 408
JO - Epigenomics
JF - Epigenomics
SN - 1750-1911
IS - 4
ER -
ID: 198721240