Developmental changes of rRNA ribose methylations in the mouse
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Developmental changes of rRNA ribose methylations in the mouse. / Hebras, Jade; Krogh, Nicolai; Marty, Virginie; Nielsen, Henrik; Cavaillé, Jérôme.
I: RNA Biology, Bind 17, Nr. 1, 2020, s. 150-164.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Developmental changes of rRNA ribose methylations in the mouse
AU - Hebras, Jade
AU - Krogh, Nicolai
AU - Marty, Virginie
AU - Nielsen, Henrik
AU - Cavaillé, Jérôme
PY - 2020
Y1 - 2020
N2 - A sequencing-based profiling method (RiboMeth-seq) for ribose methylations was used to study methylation patterns in mouse adult tissues and during development. In contrast to previous reports based on studies of human cancer cell lines, almost all methylation sites were close to fully methylated in adult tissues. A subset of sites was differentially modified in developing tissues compared to their adult counterparts and showed clear developmental dynamics. This provides the first evidence for ribosome heterogeneity at the level of rRNA modifications during mouse development. In a prominent example, the expression levels of SNORD78 during development appeared to be regulated by alternative splicing of the Gas5 host-gene and to correlate with the methylation level of its target site at LSU-G4593. The results are discussed in the context of the specialized ribosome hypothesis.
AB - A sequencing-based profiling method (RiboMeth-seq) for ribose methylations was used to study methylation patterns in mouse adult tissues and during development. In contrast to previous reports based on studies of human cancer cell lines, almost all methylation sites were close to fully methylated in adult tissues. A subset of sites was differentially modified in developing tissues compared to their adult counterparts and showed clear developmental dynamics. This provides the first evidence for ribosome heterogeneity at the level of rRNA modifications during mouse development. In a prominent example, the expression levels of SNORD78 during development appeared to be regulated by alternative splicing of the Gas5 host-gene and to correlate with the methylation level of its target site at LSU-G4593. The results are discussed in the context of the specialized ribosome hypothesis.
U2 - 10.1080/15476286.2019.1670598
DO - 10.1080/15476286.2019.1670598
M3 - Journal article
C2 - 31566069
VL - 17
SP - 150
EP - 164
JO - R N A Biology
JF - R N A Biology
SN - 1547-6286
IS - 1
ER -
ID: 228734348