Detection of inhomogeneitis in membrain ohmic resistance in geometrycally complex systems
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Detection of inhomogeneitis in membrain ohmic resistance in geometrycally complex systems. / Svirskis, G.; Hounsgaard, J.; Gutman, A.
I: Biologicheskie Membrany, Bind 17, Nr. 3, 01.12.2000, s. 324-329.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Detection of inhomogeneitis in membrain ohmic resistance in geometrycally complex systems
AU - Svirskis, G.
AU - Hounsgaard, J.
AU - Gutman, A.
PY - 2000/12/1
Y1 - 2000/12/1
N2 - DC field-evoked transients in arbitrary shaped neurons and syncytia were analyzed theoretically. In systems with homogeneous passive membrane properties the transients develop much faster than membrane discharges. The conduction of the proximal membrane could be larger due to the injury imposed by sharp electrode impalement. In this case, the transients have overshoot and undershoot when the field is switched on and off. The overshoot and undershoot decay with the time-constant of the response to the current injection. If conductance of the distal membrane is larger, then the fast transients develop only partially and have slow tails that decay according to the time-constant of the response to current injection. We recorded DC field-evoked potentials in motoneurons in turtle spinal cord slices by sharp electrodes and in the whole-cell mode. All three theoretically predicted types of responses were observed. We have found that sharp electrodes do not impose shunt in 60% of recorded cells. Detection of various membrane inhomogeneities in 1D-syncythium is discussed. We suppose also that it is possible to detect the inhomogeneities in intercellular resistance of the syncytium and intracellular resistance of a neuron when the membrane passive properties are homogeneous.
AB - DC field-evoked transients in arbitrary shaped neurons and syncytia were analyzed theoretically. In systems with homogeneous passive membrane properties the transients develop much faster than membrane discharges. The conduction of the proximal membrane could be larger due to the injury imposed by sharp electrode impalement. In this case, the transients have overshoot and undershoot when the field is switched on and off. The overshoot and undershoot decay with the time-constant of the response to the current injection. If conductance of the distal membrane is larger, then the fast transients develop only partially and have slow tails that decay according to the time-constant of the response to current injection. We recorded DC field-evoked potentials in motoneurons in turtle spinal cord slices by sharp electrodes and in the whole-cell mode. All three theoretically predicted types of responses were observed. We have found that sharp electrodes do not impose shunt in 60% of recorded cells. Detection of various membrane inhomogeneities in 1D-syncythium is discussed. We suppose also that it is possible to detect the inhomogeneities in intercellular resistance of the syncytium and intracellular resistance of a neuron when the membrane passive properties are homogeneous.
UR - http://www.scopus.com/inward/record.url?scp=0039763619&partnerID=8YFLogxK
M3 - Journal article
AN - SCOPUS:0039763619
VL - 17
SP - 324
EP - 329
JO - Biologicheskie Membrany
JF - Biologicheskie Membrany
SN - 0233-4755
IS - 3
ER -
ID: 237698898