Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion. / Pettygrove, Brian A.; Kratofil, Rachel M.; Alhede, Maria; Jensen, Peter; Newton, Michelle; Qvortrup, Klaus; Pallister, Kyler B.; Bjarnsholt, Thomas; Kubes, Paul; Voyich, Jovanka M.; Stewart, Philip S.
I: Biomaterials, Bind 275, 120775, 08.2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Delayed neutrophil recruitment allows nascent Staphylococcus aureus biofilm formation and immune evasion
AU - Pettygrove, Brian A.
AU - Kratofil, Rachel M.
AU - Alhede, Maria
AU - Jensen, Peter
AU - Newton, Michelle
AU - Qvortrup, Klaus
AU - Pallister, Kyler B.
AU - Bjarnsholt, Thomas
AU - Kubes, Paul
AU - Voyich, Jovanka M.
AU - Stewart, Philip S.
N1 - Publisher Copyright: © 2021 Elsevier Ltd
PY - 2021/8
Y1 - 2021/8
N2 - Biofilms that form on implanted medical devices cause recalcitrant infections. The early events enabling contaminating bacteria to evade immune clearance, before a mature biofilm is established, are poorly understood. Live imaging in vitro demonstrated that Staphylococcus aureus sparsely inoculated on an abiotic surface can go undiscovered by human neutrophils, grow, and form aggregates. Small (~50 μm2) aggregates of attached bacteria resisted killing by human neutrophils, resulting in neutrophil lysis and bacterial persistence. In vivo, neutrophil recruitment to a peritoneal implant was spatially heterogenous, with some bacterial aggregates remaining undiscovered by neutrophils after 24 h. Intravital imaging in mouse skin revealed that attached S. aureus aggregates grew and remained undiscovered by neutrophils for up to 3 h. These results suggest a model in which delayed recruitment of neutrophils to an abiotic implant presents a critical window in which bacteria establish a nascent biofilm and acquire tolerance to neutrophil killing.
AB - Biofilms that form on implanted medical devices cause recalcitrant infections. The early events enabling contaminating bacteria to evade immune clearance, before a mature biofilm is established, are poorly understood. Live imaging in vitro demonstrated that Staphylococcus aureus sparsely inoculated on an abiotic surface can go undiscovered by human neutrophils, grow, and form aggregates. Small (~50 μm2) aggregates of attached bacteria resisted killing by human neutrophils, resulting in neutrophil lysis and bacterial persistence. In vivo, neutrophil recruitment to a peritoneal implant was spatially heterogenous, with some bacterial aggregates remaining undiscovered by neutrophils after 24 h. Intravital imaging in mouse skin revealed that attached S. aureus aggregates grew and remained undiscovered by neutrophils for up to 3 h. These results suggest a model in which delayed recruitment of neutrophils to an abiotic implant presents a critical window in which bacteria establish a nascent biofilm and acquire tolerance to neutrophil killing.
KW - Biofilm
KW - Implant-associated infection
KW - Microscopy
KW - Neutrophil
KW - Staphylococcus aureus
KW - Up to 10)
U2 - 10.1016/j.biomaterials.2021.120775
DO - 10.1016/j.biomaterials.2021.120775
M3 - Journal article
C2 - 34243039
AN - SCOPUS:85109215098
VL - 275
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
M1 - 120775
ER -
ID: 274569161