Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation
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Post-transcriptional gene regulation in T cells is dynamic and complex as targeted transcripts respond to various factors. This is evident for the Icos mRNA encoding an essential costimulatory receptor that is regulated by several RNA-binding proteins (RBP), including Roquin-1 and Roquin-2. Here, we identify a core RBPome of 798 mouse and 801 human T cell proteins by utilizing global RNA interactome capture (RNA-IC) and orthogonal organic phase separation (OOPS). The RBPome includes Stat1, Stat4 and Vav1 proteins suggesting unexpected functions for these transcription factors and signal transducers. Based on proximity to Roquin-1, we select ~50 RBPs for testing coregulation of Roquin-1/2 targets by induced expression in wild-type or Roquin-1/2-deficient T cells. Besides Roquin-independent contributions from Rbms1 and Cpeb4 we also show Roquin-1/2-dependent and target-specific coregulation of Icos by Celf1 and Igf2bp3. Connecting the cellular RBPome in a post-transcriptional context, we find contributions from multiple RBPs to the prototypic regulation of mRNA targets by individual trans-acting factors.
Originalsprog | Engelsk |
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Tidsskrift | Nature Communications |
Vol/bind | 12 |
Udgave nummer | 1 |
Sider (fra-til) | 5208 |
ISSN | 2041-1723 |
DOI | |
Status | Udgivet - 1 sep. 2021 |
Eksternt udgivet | Ja |
Bibliografisk note
© 2021. The Author(s).
ID: 303116308