Decellularization of the Murine Cardiopulmonary Complex
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Decellularization of the Murine Cardiopulmonary Complex. / Mayorca-Guiliani, Alejandro E.; Rafaeva, Maria; Willacy, Oliver; Madsen, Chris D.; Reuten, Raphael; Erler, Janine T.
I: Journal of Visualized Experiments, Bind 171, e61854, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Decellularization of the Murine Cardiopulmonary Complex
AU - Mayorca-Guiliani, Alejandro E.
AU - Rafaeva, Maria
AU - Willacy, Oliver
AU - Madsen, Chris D.
AU - Reuten, Raphael
AU - Erler, Janine T.
PY - 2021
Y1 - 2021
N2 - We present here a decellularization protocol for mouse heart and lungs. It produces structural ECM scaffolds that can be used to analyze ECM topology and composition. It is based on a microsurgical procedure designed to catheterize the trachea and aorta of a euthanized mouse to perfuse the heart and lungs with decellularizing agents. The decellularized cardiopulmonary complex can subsequently be immunostained to reveal the location of structural ECM proteins. The whole procedure can be completed in 4 days.,The ECM scaffolds resulting from this protocol are free of dimensional distortions. The absence of cells enables structural examination of ECM structures down to submicron resolution in 3D. This protocol can be applied to healthy and diseased tissue from mice as young as 4-weeks old, including mouse models of fibrosis and cancer, opening the way to determine ECM remodeling associated with cardiopulmonary disease.
AB - We present here a decellularization protocol for mouse heart and lungs. It produces structural ECM scaffolds that can be used to analyze ECM topology and composition. It is based on a microsurgical procedure designed to catheterize the trachea and aorta of a euthanized mouse to perfuse the heart and lungs with decellularizing agents. The decellularized cardiopulmonary complex can subsequently be immunostained to reveal the location of structural ECM proteins. The whole procedure can be completed in 4 days.,The ECM scaffolds resulting from this protocol are free of dimensional distortions. The absence of cells enables structural examination of ECM structures down to submicron resolution in 3D. This protocol can be applied to healthy and diseased tissue from mice as young as 4-weeks old, including mouse models of fibrosis and cancer, opening the way to determine ECM remodeling associated with cardiopulmonary disease.
U2 - 10.3791/61854
DO - 10.3791/61854
M3 - Journal article
C2 - 34125099
VL - 171
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
SN - 1940-087X
M1 - e61854
ER -
ID: 272401274