CXCR3 expression and activation of eosinophils: role of IFN-gamma-inducible protein-10 and monokine induced by IFN-gamma
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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CXCR3 expression and activation of eosinophils : role of IFN-gamma-inducible protein-10 and monokine induced by IFN-gamma. / Jinquan, T; Jing, C; Jacobi, H H; Reimert, C M; Millner, A; Quan, S; Hansen, J B; Dissing, S; Malling, H J; Skov, P S; Poulsen, L K.
I: Journal of immunology (Baltimore, Md. : 1950), Bind 165, Nr. 3, 01.08.2000, s. 1548-56.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - CXCR3 expression and activation of eosinophils
T2 - role of IFN-gamma-inducible protein-10 and monokine induced by IFN-gamma
AU - Jinquan, T
AU - Jing, C
AU - Jacobi, H H
AU - Reimert, C M
AU - Millner, A
AU - Quan, S
AU - Hansen, J B
AU - Dissing, S
AU - Malling, H J
AU - Skov, P S
AU - Poulsen, L K
PY - 2000/8/1
Y1 - 2000/8/1
N2 - CXC chemokine receptor 3 (CXCR3), predominately expressed on memory/activated T lymphocytes, is a receptor for both IFN-gamma-inducible protein-10 (gamma IP-10) and monokine induced by IFN-gamma (Mig). We report a novel finding that CXCR3 is also expressed on eosinophils. gamma IP-10 and Mig induce eosinophil chemotaxis via CXCR3, as documented by the fact that anti-CXCR3 mAb blocks gamma IP-10- and Mig-induced eosinophil chemotaxis. gamma IP-10- and Mig-induced eosinophil chemotaxis are up- and down-regulated by IL-2 and IL-10, respectively. Correspondingly, CXCR3 protein and mRNA expressions in eosinophils are up- and down-regulated by IL-2 and IL-10, respectively, as detected using flow cytometry, immunocytochemical assay, and a real-time quantitative RT-PCR technique. gamma IP-10 and Mig act eosinophils to induce chemotaxis via the cAMP-dependent protein kinase A signaling pathways. The fact that gamma IP-10 and Mig induce an increase in intracellular calcium in eosinophils confirms that CXCR3 exists on eosinophils. Besides induction to chemotaxis, gamma IP-10 and Mig also activate eosinophils to eosinophil cationic protein release. These results indicate that CXCR3-gamma IP-10 and -Mig receptor-ligand pairs as well as the effects of IL-2 and IL-10 on them may be especially important in the cytokine/chemokine environment for the pathophysiologic events of allergic inflammation, including initiation, progression, and termination in the processes.
AB - CXC chemokine receptor 3 (CXCR3), predominately expressed on memory/activated T lymphocytes, is a receptor for both IFN-gamma-inducible protein-10 (gamma IP-10) and monokine induced by IFN-gamma (Mig). We report a novel finding that CXCR3 is also expressed on eosinophils. gamma IP-10 and Mig induce eosinophil chemotaxis via CXCR3, as documented by the fact that anti-CXCR3 mAb blocks gamma IP-10- and Mig-induced eosinophil chemotaxis. gamma IP-10- and Mig-induced eosinophil chemotaxis are up- and down-regulated by IL-2 and IL-10, respectively. Correspondingly, CXCR3 protein and mRNA expressions in eosinophils are up- and down-regulated by IL-2 and IL-10, respectively, as detected using flow cytometry, immunocytochemical assay, and a real-time quantitative RT-PCR technique. gamma IP-10 and Mig act eosinophils to induce chemotaxis via the cAMP-dependent protein kinase A signaling pathways. The fact that gamma IP-10 and Mig induce an increase in intracellular calcium in eosinophils confirms that CXCR3 exists on eosinophils. Besides induction to chemotaxis, gamma IP-10 and Mig also activate eosinophils to eosinophil cationic protein release. These results indicate that CXCR3-gamma IP-10 and -Mig receptor-ligand pairs as well as the effects of IL-2 and IL-10 on them may be especially important in the cytokine/chemokine environment for the pathophysiologic events of allergic inflammation, including initiation, progression, and termination in the processes.
KW - Blood Proteins
KW - Calcium
KW - Chemokine CXCL10
KW - Chemokine CXCL9
KW - Chemokines, CXC
KW - Chemotaxis, Leukocyte
KW - Eosinophil Granule Proteins
KW - Eosinophils
KW - Humans
KW - Inflammation Mediators
KW - Intercellular Signaling Peptides and Proteins
KW - Interferon-gamma
KW - Interleukin-10
KW - Interleukin-2
KW - Intracellular Fluid
KW - Ligands
KW - Receptors, CXCR3
KW - Receptors, Chemokine
KW - Receptors, Cytokine
KW - Ribonucleases
KW - Signal Transduction
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Journal article
C2 - 10903763
VL - 165
SP - 1548
EP - 1556
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 3
ER -
ID: 169715932