Correlation between HPV status at T and N sites of oropharyngeal squamous cell carcinomas
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Correlation between HPV status at T and N sites of oropharyngeal squamous cell carcinomas. / Josiassen, Michael Vallop; Charabi, Birgitte; Lajer, Christel Braemer; Garnaes, Emilie; Hamilton Therkildsen, Marianne; Norrild, Bodil; Specht, Lena; Nielsen, Finn Cilius; von Buchwald, Christian.
I: Acta Oto-Laryngologica, Bind 137, Nr. 12, 12.2017, s. 1260-1264.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Correlation between HPV status at T and N sites of oropharyngeal squamous cell carcinomas
AU - Josiassen, Michael Vallop
AU - Charabi, Birgitte
AU - Lajer, Christel Braemer
AU - Garnaes, Emilie
AU - Hamilton Therkildsen, Marianne
AU - Norrild, Bodil
AU - Specht, Lena
AU - Nielsen, Finn Cilius
AU - von Buchwald, Christian
PY - 2017/12
Y1 - 2017/12
N2 - Objectives: Human papilloma virus (HPV) is known to be associated with oropharyngeal squamous cell carcinomas (OPSCC) and may potentially play a vital role in tumor metastasis. The purpose of this study was to correlate HPV status of cervical lymph node metastases with their respective primary OPSCC tumor. Methods: Formalin-fixed, paraffin-embedded (FFPE) tissue samples obtained from 34 patients with cervical lymph node metastases were analyzed with HPV 16 DNA polymerase chain reaction (PCR), p16 immunohistochemistry and HPV typing. The results were correlated with the HPV status and type found in the primary tumors of OPSCC. Results: Comparing HPV DNA status with p16 we found that 21 primary tumors and lymph node metastases were HPV positive (61.8%) and seven primary tumors and lymph node metastases were HPV negative (20.6%). Six patient samples differed when correlating the primary tumor and lymph node metastasis (17.6%). Conclusions: In this study, HPV status in OPSCCs and their cervical lymph node metastases correlated in the vast majority of cases. However, HPV detection methods may have certain limitations resulting in varying degree of non-correlation. This should be taken into account when stratifying treatment in regard to HPV status.
AB - Objectives: Human papilloma virus (HPV) is known to be associated with oropharyngeal squamous cell carcinomas (OPSCC) and may potentially play a vital role in tumor metastasis. The purpose of this study was to correlate HPV status of cervical lymph node metastases with their respective primary OPSCC tumor. Methods: Formalin-fixed, paraffin-embedded (FFPE) tissue samples obtained from 34 patients with cervical lymph node metastases were analyzed with HPV 16 DNA polymerase chain reaction (PCR), p16 immunohistochemistry and HPV typing. The results were correlated with the HPV status and type found in the primary tumors of OPSCC. Results: Comparing HPV DNA status with p16 we found that 21 primary tumors and lymph node metastases were HPV positive (61.8%) and seven primary tumors and lymph node metastases were HPV negative (20.6%). Six patient samples differed when correlating the primary tumor and lymph node metastasis (17.6%). Conclusions: In this study, HPV status in OPSCCs and their cervical lymph node metastases correlated in the vast majority of cases. However, HPV detection methods may have certain limitations resulting in varying degree of non-correlation. This should be taken into account when stratifying treatment in regard to HPV status.
KW - cervical lymph node metastases
KW - HPV
KW - oropharyngeal cancer
KW - p16
KW - squamous cell carcinoma
U2 - 10.1080/00016489.2017.1356934
DO - 10.1080/00016489.2017.1356934
M3 - Journal article
C2 - 28758552
AN - SCOPUS:85026551127
VL - 137
SP - 1260
EP - 1264
JO - Acta Oto-Laryngologica, Supplement
JF - Acta Oto-Laryngologica, Supplement
SN - 0365-5237
IS - 12
ER -
ID: 185748216