Conformational fluctuations affect protein alignment in dilute liquid crystal media.
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Conformational fluctuations affect protein alignment in dilute liquid crystal media. / Louhivuori, M.; Otten, R.; Lindorff-Larsen, Kresten; Annila, A.
I: Journal of the American Chemical Society, Bind 128, Nr. 13, 2006, s. 4371-4376.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Conformational fluctuations affect protein alignment in dilute liquid crystal media.
AU - Louhivuori, M.
AU - Otten, R.
AU - Lindorff-Larsen, Kresten
AU - Annila, A.
PY - 2006
Y1 - 2006
N2 - The discovery of dilute liquid crystalline media to align biological macromolecules has opened many new possibilities to study protein and nucleic acid structures by NMR spectroscopy. We inspect the basic alignment phenomenon for an ensemble of protein conformations to deduce relative contributions of each member to the residual dipolar coupling signals. We find that molecular fluctuations can affect the alignment and discover a resulting emphasis of certain conformations. However, the internal fluctuations are largely uncorrelated with those of the alignment, implying that proteins have liquidlike molecular surfaces. Furthermore, we consider the implications of a dynamic bias to structure determination using data from the weak alignment method.
AB - The discovery of dilute liquid crystalline media to align biological macromolecules has opened many new possibilities to study protein and nucleic acid structures by NMR spectroscopy. We inspect the basic alignment phenomenon for an ensemble of protein conformations to deduce relative contributions of each member to the residual dipolar coupling signals. We find that molecular fluctuations can affect the alignment and discover a resulting emphasis of certain conformations. However, the internal fluctuations are largely uncorrelated with those of the alignment, implying that proteins have liquidlike molecular surfaces. Furthermore, we consider the implications of a dynamic bias to structure determination using data from the weak alignment method.
U2 - 10.1021/ja0576334
DO - 10.1021/ja0576334
M3 - Journal article
VL - 128
SP - 4371
EP - 4376
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 13
ER -
ID: 1099769