Comparison of Cytokines in Skin Biopsies and Tape Strips from Adults with Atopic Dermatitis
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Skin biomarkers for disease severity and treatment response in atopic dermatitis (AD) are needed. Biopsies cause scarring and tape stripping represents an alternative minimally invasive method for stratum corneum sampling. In this study, we examined the gene expression of cytokines in skin biopsies and cytokines in stratum corneum tape strips collected from adults with AD. We collected punch biopsies and tape strips from healthy controls (n = 6) and subjects with AD (n = 12) at baseline and after 2 weeks of topical treatment with mometasone furoate 0.1% cream. We found that IFN-γ, IL-13, and IL-10 mRNA (biopsies) and IL-1β protein expression levels (tape strips) were significantly increased in lesional AD skin compared to healthy control skin. Treatment with topical corticosteroid led to a significant decrease in mRNA levels for IL-13 and IL-4R but no significant differences in cytokine protein levels measured in tape strips. Finally, we found no significant correlations between cytokine levels in tape strips and mRNA levels in skin biopsies.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Dermatology |
| Vol/bind | 237 |
| Udgave nummer | 6 |
| Sider (fra-til) | 940-945 |
| Antal sider | 6 |
| ISSN | 1018-8665 |
| DOI | |
| Status | Udgivet - 2021 |
Bibliografisk note
Funding Information:
The study presented here was made possible by financial support from Sanofi-Aventis Denmark. Sanofi-Aventis was not involved in any part of the study design, data collection, data analysis, manuscript preparation or review process, or in the decision to submit the manuscript for publication.
Funding Information:
S.S., P.B., and S.K. have no conflicts of interest to declare. L.S. has been a paid speaker for AbbVie, Eli Lilly, Novartis, Sanofi and LEO Pharma, and has been a consultant or has served on Advisory Boards with AbbVie, Janssen Cilag, Novartis, Eli Lilly, LEO Pharma, UCB, Almirall, and Sanofi. She has served as an investigator for AbbVie, Sanofi, Janssen Cilag, Boehringer Ingelheim, As-traZenica, Eli Lilly, Novartis, Pfizer, Regeneron, and LEO Pharma, and has received research and educational grants from Novartis, Sanofi, Janssen Cilag, and LEO Pharma. J.P.T. has been an investigator/speaker or advisor for Abbvie, Pfizer, LEO Pharma, Eli Lilly & Co, Regerenon, and Sanofi-Genzyme.
Publisher Copyright:
© 2021 S. Karger AG. All rights reserved.
ID: 304364167