Collagen-Elastin and Collagen-Glycosaminoglycan Scaffolds Promote Distinct Patterns of Matrix Maturation and Axial Vascularization in Arteriovenous Loop-Based Soft Tissue Flaps
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Collagen-Elastin and Collagen-Glycosaminoglycan Scaffolds Promote Distinct Patterns of Matrix Maturation and Axial Vascularization in Arteriovenous Loop-Based Soft Tissue Flaps. / Schmidt, Volker J; Wietbrock, Johanna O; Leibig, Nico; Gloe, Torsten; Henn, Dominic; Hernekamp, J Frederik; Harhaus, Leila; Kneser, Ulrich.
I: Annals of Plastic Surgery, Bind 79, Nr. 1, 07.2017, s. 92-100.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Collagen-Elastin and Collagen-Glycosaminoglycan Scaffolds Promote Distinct Patterns of Matrix Maturation and Axial Vascularization in Arteriovenous Loop-Based Soft Tissue Flaps
AU - Schmidt, Volker J
AU - Wietbrock, Johanna O
AU - Leibig, Nico
AU - Gloe, Torsten
AU - Henn, Dominic
AU - Hernekamp, J Frederik
AU - Harhaus, Leila
AU - Kneser, Ulrich
PY - 2017/7
Y1 - 2017/7
N2 - INTRODUCTION: Autologous free flaps are the criterion standard for reconstructions of complex soft tissue defects; however, they are limited by donor-site morbidities. The arteriovenous (AV) loop model enables the generation of soft tissue constructs based on acellular dermal matrices with a functional microvasculature and minimal donor site morbidity. The ideal scaffold for AV loop-based tissue engineering has not been determined.METHODS: AV loops were placed into subcutaneous isolation chambers filled with either a collagen-elastin scaffold or a collagen-glycosaminoglycan scaffold in the thighs of rats. Matrix elasticity, neoangiogenesis, cell migration, and proliferation were compared after 14 and 28 days.RESULTS: Mean vessel count and area had increased in both matrices at 28 compared with 14 days. Collagen-elastin matrices showed a higher mean vessel count and area compared with collagen-glycosaminoglycan matrices at 14 days. At 28 days, a more homogeneous vascular network and higher cell counts were observed in collagen-elastin matrices. Collagen-glycosaminoglycan matrices, however, exhibited less volume loss at day 28.CONCLUSIONS: Collagen-based scaffolds are suitable for soft tissue engineering in conjunction with the AV loop technique. These scaffolds exhibit distinct patterns of angiogenesis, cell migration, and proliferation and may in the future serve as the basis of tissue-engineered free flaps as an individualized treatment concept for critical wounds.
AB - INTRODUCTION: Autologous free flaps are the criterion standard for reconstructions of complex soft tissue defects; however, they are limited by donor-site morbidities. The arteriovenous (AV) loop model enables the generation of soft tissue constructs based on acellular dermal matrices with a functional microvasculature and minimal donor site morbidity. The ideal scaffold for AV loop-based tissue engineering has not been determined.METHODS: AV loops were placed into subcutaneous isolation chambers filled with either a collagen-elastin scaffold or a collagen-glycosaminoglycan scaffold in the thighs of rats. Matrix elasticity, neoangiogenesis, cell migration, and proliferation were compared after 14 and 28 days.RESULTS: Mean vessel count and area had increased in both matrices at 28 compared with 14 days. Collagen-elastin matrices showed a higher mean vessel count and area compared with collagen-glycosaminoglycan matrices at 14 days. At 28 days, a more homogeneous vascular network and higher cell counts were observed in collagen-elastin matrices. Collagen-glycosaminoglycan matrices, however, exhibited less volume loss at day 28.CONCLUSIONS: Collagen-based scaffolds are suitable for soft tissue engineering in conjunction with the AV loop technique. These scaffolds exhibit distinct patterns of angiogenesis, cell migration, and proliferation and may in the future serve as the basis of tissue-engineered free flaps as an individualized treatment concept for critical wounds.
KW - Acellular Dermis/drug effects
KW - Animals
KW - Collagen/pharmacology
KW - Disease Models, Animal
KW - Elastin/pharmacology
KW - Female
KW - Glycosaminoglycans/pharmacology
KW - Graft Survival
KW - Microvessels/drug effects
KW - Neovascularization, Physiologic/drug effects
KW - Random Allocation
KW - Rats
KW - Rats, Sprague-Dawley
KW - Reference Values
KW - Sensitivity and Specificity
KW - Surgical Flaps/blood supply
KW - Tissue Engineering/methods
KW - Tissue Scaffolds
KW - Wound Healing/drug effects
U2 - 10.1097/SAP.0000000000001096
DO - 10.1097/SAP.0000000000001096
M3 - Journal article
C2 - 28542070
VL - 79
SP - 92
EP - 100
JO - Annals of Plastic Surgery
JF - Annals of Plastic Surgery
SN - 0148-7043
IS - 1
ER -
ID: 329566823