Collagen Growth Pattern in Human Articular Cartilage of the Knee
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Collagen Growth Pattern in Human Articular Cartilage of the Knee. / Jørgensen, Adam E.M.; Schjerling, Peter; Krogsgaard, Michael R.; Petersen, Michael M.; Olsen, Jesper; Kjær, Michael; Heinemeier, Katja M.
I: Cartilage, Bind 13, Nr. Suppl. 2, 2021, s. 408S-418S.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Collagen Growth Pattern in Human Articular Cartilage of the Knee
AU - Jørgensen, Adam E.M.
AU - Schjerling, Peter
AU - Krogsgaard, Michael R.
AU - Petersen, Michael M.
AU - Olsen, Jesper
AU - Kjær, Michael
AU - Heinemeier, Katja M.
N1 - Publisher Copyright: © The Author(s) 2020.
PY - 2021
Y1 - 2021
N2 - Objective: During skeletal growth, the articular cartilage expands to maintain its cover of bones in joints, however, it is unclear when and how cartilage grows. We aim to determine the expanding growth pattern and timing across the tibia plateau in human knees. Design: Six human tibia plateaus (2 healthy, 2 with osteoarthritis, and 2 with posttraumatic osteoarthritis) were used for full-depth cartilage sampling systematically across the joint surface at 12 medial and 4 lateral sites. Methodologically, we took advantage of the performed nuclear bomb tests in the years 1955 to 1963, which increased the atmospheric 14C that was incorporated into human tissues. Cartilage was treated enzymatically to extract collagen, analyzed for 14C content, and year at formation was determined from historical atmospheric 14C concentrations. Results: By age-determination, each tibia condyle had central points of formation surrounded by later-formed cartilage toward the periphery. Furthermore, the tibia plateaus contained collagen with 14C levels corresponding to mean donor age of 11.7 years (±3.8 SD). Finally, the medial condyle had lower 14C levels corresponding to formation 1 year later than the lateral condyle (P = 0.009). Conclusions: Human cartilage on the tibia plateau contains collagen that has experienced little if any turnover since school-age. The cartilage formation develops from 2 condyle centers and radially outward with the medial condyle finishing slightly later than the lateral condyle. This suggests a childhood programmed cartilage formation with a very limited adulthood collagen turnover.
AB - Objective: During skeletal growth, the articular cartilage expands to maintain its cover of bones in joints, however, it is unclear when and how cartilage grows. We aim to determine the expanding growth pattern and timing across the tibia plateau in human knees. Design: Six human tibia plateaus (2 healthy, 2 with osteoarthritis, and 2 with posttraumatic osteoarthritis) were used for full-depth cartilage sampling systematically across the joint surface at 12 medial and 4 lateral sites. Methodologically, we took advantage of the performed nuclear bomb tests in the years 1955 to 1963, which increased the atmospheric 14C that was incorporated into human tissues. Cartilage was treated enzymatically to extract collagen, analyzed for 14C content, and year at formation was determined from historical atmospheric 14C concentrations. Results: By age-determination, each tibia condyle had central points of formation surrounded by later-formed cartilage toward the periphery. Furthermore, the tibia plateaus contained collagen with 14C levels corresponding to mean donor age of 11.7 years (±3.8 SD). Finally, the medial condyle had lower 14C levels corresponding to formation 1 year later than the lateral condyle (P = 0.009). Conclusions: Human cartilage on the tibia plateau contains collagen that has experienced little if any turnover since school-age. The cartilage formation develops from 2 condyle centers and radially outward with the medial condyle finishing slightly later than the lateral condyle. This suggests a childhood programmed cartilage formation with a very limited adulthood collagen turnover.
KW - 14C
KW - articular cartilage
KW - collagen
KW - development
KW - radiocarbon dating
KW - tissue
U2 - 10.1177/1947603520971016
DO - 10.1177/1947603520971016
M3 - Journal article
C2 - 33147986
AN - SCOPUS:85095429345
VL - 13
SP - 408S-418S
JO - Cartilage
JF - Cartilage
SN - 1947-6035
IS - Suppl. 2
ER -
ID: 286628302