Closing in on the C. elegans ORFeome by cloning TWINSCAN predictions
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Closing in on the C. elegans ORFeome by cloning TWINSCAN predictions. / Wei, Chaochun; Lamesch, Philippe; Arumugam, Manimozhiyan; Rosenberg, Jennifer; Hu, Ping; Vidal, Marc; Brent, Michael R.
I: Genome Research, Bind 15, Nr. 4, 2005, s. 577-82.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Closing in on the C. elegans ORFeome by cloning TWINSCAN predictions
AU - Wei, Chaochun
AU - Lamesch, Philippe
AU - Arumugam, Manimozhiyan
AU - Rosenberg, Jennifer
AU - Hu, Ping
AU - Vidal, Marc
AU - Brent, Michael R
PY - 2005
Y1 - 2005
N2 - The genome of Caenorhabditis elegans was the first animal genome to be sequenced. Although considerable effort has been devoted to annotating it, the standard WormBase annotation contains thousands of predicted genes for which there is no cDNA or EST evidence. We hypothesized that a more complete experimental annotation could be obtained by creating a more accurate gene-prediction program and then amplifying and sequencing predicted genes. Our approach was to adapt the TWINSCAN gene prediction system to C. elegans and C. briggsae and to improve its splice site and intron-length models. The resulting system has 60% sensitivity and 58% specificity in exact prediction of open reading frames (ORFs), and hence, proteins-the best results we are aware of any multicellular organism. We then attempted to amplify, clone, and sequence 265 TWINSCAN-predicted ORFs that did not overlap WormBase gene annotations. The success rate was 55%, adding 146 genes that were completely absent from WormBase to the ORF clone collection (ORFeome). The same procedure had a 7% success rate on 90 Worm Base "predicted" genes that do not overlap TWINSCAN predictions. These results indicate that the accuracy of WormBase could be significantly increased by replacing its partially curated predicted genes with TWINSCAN predictions. The technology described in this study will continue to drive the C. elegans ORFeome toward completion and contribute to the annotation of the three Caenorhabditis species currently being sequenced. The results also suggest that this technology can significantly improve our knowledge of the "parts list" for even the best-studied model organisms.
AB - The genome of Caenorhabditis elegans was the first animal genome to be sequenced. Although considerable effort has been devoted to annotating it, the standard WormBase annotation contains thousands of predicted genes for which there is no cDNA or EST evidence. We hypothesized that a more complete experimental annotation could be obtained by creating a more accurate gene-prediction program and then amplifying and sequencing predicted genes. Our approach was to adapt the TWINSCAN gene prediction system to C. elegans and C. briggsae and to improve its splice site and intron-length models. The resulting system has 60% sensitivity and 58% specificity in exact prediction of open reading frames (ORFs), and hence, proteins-the best results we are aware of any multicellular organism. We then attempted to amplify, clone, and sequence 265 TWINSCAN-predicted ORFs that did not overlap WormBase gene annotations. The success rate was 55%, adding 146 genes that were completely absent from WormBase to the ORF clone collection (ORFeome). The same procedure had a 7% success rate on 90 Worm Base "predicted" genes that do not overlap TWINSCAN predictions. These results indicate that the accuracy of WormBase could be significantly increased by replacing its partially curated predicted genes with TWINSCAN predictions. The technology described in this study will continue to drive the C. elegans ORFeome toward completion and contribute to the annotation of the three Caenorhabditis species currently being sequenced. The results also suggest that this technology can significantly improve our knowledge of the "parts list" for even the best-studied model organisms.
KW - Animals
KW - Caenorhabditis elegans
KW - Cloning, Molecular
KW - Computational Biology
KW - Databases, Genetic
KW - Genes, Helminth
KW - Genome
KW - Genomics
KW - Introns
KW - Open Reading Frames
KW - Sensitivity and Specificity
U2 - 10.1101/gr.3329005
DO - 10.1101/gr.3329005
M3 - Journal article
C2 - 15805498
VL - 15
SP - 577
EP - 582
JO - Genome Research
JF - Genome Research
SN - 1088-9051
IS - 4
ER -
ID: 43976106