Chronic p53-independent p21 expression causes genomic instability by deregulating replication licensing

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Standard

Chronic p53-independent p21 expression causes genomic instability by deregulating replication licensing. / Galanos, Panagiotis; Vougas, Konstantinos; Walter, David; Polyzos, Alexander; Maya-Mendoza, Apolinar; Haagensen, Emma J; Kokkalis, Antonis; Roumelioti, Fani-Marlen; Gagos, Sarantis; Tzetis, Maria; Canovas, Begoña; Igea, Ana; Ahuja, Akshay K; Zellweger, Ralph; Havaki, Sofia; Kanavakis, Emanuel; Kletsas, Dimitris; Roninson, Igor B; Garbis, Spiros D; Lopes, Massimo; Nebreda, Angel; Thanos, Dimitris; Blow, J Julian; Townsend, Paul; Sørensen, Claus Storgaard; Bartek, Jiri; Gorgoulis, Vassilis G.

I: Nature Cell Biology, Bind 18, Nr. 7, 07.2016, s. 777-89.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Galanos, P, Vougas, K, Walter, D, Polyzos, A, Maya-Mendoza, A, Haagensen, EJ, Kokkalis, A, Roumelioti, F-M, Gagos, S, Tzetis, M, Canovas, B, Igea, A, Ahuja, AK, Zellweger, R, Havaki, S, Kanavakis, E, Kletsas, D, Roninson, IB, Garbis, SD, Lopes, M, Nebreda, A, Thanos, D, Blow, JJ, Townsend, P, Sørensen, CS, Bartek, J & Gorgoulis, VG 2016, 'Chronic p53-independent p21 expression causes genomic instability by deregulating replication licensing', Nature Cell Biology, bind 18, nr. 7, s. 777-89. https://doi.org/10.1038/ncb3378

APA

Galanos, P., Vougas, K., Walter, D., Polyzos, A., Maya-Mendoza, A., Haagensen, E. J., Kokkalis, A., Roumelioti, F-M., Gagos, S., Tzetis, M., Canovas, B., Igea, A., Ahuja, A. K., Zellweger, R., Havaki, S., Kanavakis, E., Kletsas, D., Roninson, I. B., Garbis, S. D., ... Gorgoulis, V. G. (2016). Chronic p53-independent p21 expression causes genomic instability by deregulating replication licensing. Nature Cell Biology, 18(7), 777-89. https://doi.org/10.1038/ncb3378

Vancouver

Galanos P, Vougas K, Walter D, Polyzos A, Maya-Mendoza A, Haagensen EJ o.a. Chronic p53-independent p21 expression causes genomic instability by deregulating replication licensing. Nature Cell Biology. 2016 jul.;18(7):777-89. https://doi.org/10.1038/ncb3378

Author

Galanos, Panagiotis ; Vougas, Konstantinos ; Walter, David ; Polyzos, Alexander ; Maya-Mendoza, Apolinar ; Haagensen, Emma J ; Kokkalis, Antonis ; Roumelioti, Fani-Marlen ; Gagos, Sarantis ; Tzetis, Maria ; Canovas, Begoña ; Igea, Ana ; Ahuja, Akshay K ; Zellweger, Ralph ; Havaki, Sofia ; Kanavakis, Emanuel ; Kletsas, Dimitris ; Roninson, Igor B ; Garbis, Spiros D ; Lopes, Massimo ; Nebreda, Angel ; Thanos, Dimitris ; Blow, J Julian ; Townsend, Paul ; Sørensen, Claus Storgaard ; Bartek, Jiri ; Gorgoulis, Vassilis G. / Chronic p53-independent p21 expression causes genomic instability by deregulating replication licensing. I: Nature Cell Biology. 2016 ; Bind 18, Nr. 7. s. 777-89.

Bibtex

@article{ede5cff66d8e4966910b42b8bef8f06b,
title = "Chronic p53-independent p21 expression causes genomic instability by deregulating replication licensing",
abstract = "The cyclin-dependent kinase inhibitor p21(WAF1/CIP1) (p21) is a cell-cycle checkpoint effector and inducer of senescence, regulated by p53. Yet, evidence suggests that p21 could also be oncogenic, through a mechanism that has so far remained obscure. We report that a subset of atypical cancerous cells strongly expressing p21 showed proliferation features. This occurred predominantly in p53-mutant human cancers, suggesting p53-independent upregulation of p21 selectively in more aggressive tumour cells. Multifaceted phenotypic and genomic analyses of p21-inducible, p53-null, cancerous and near-normal cellular models showed that after an initial senescence-like phase, a subpopulation of p21-expressing proliferating cells emerged, featuring increased genomic instability, aggressiveness and chemoresistance. Mechanistically, sustained p21 accumulation inhibited mainly the CRL4-CDT2 ubiquitin ligase, leading to deregulated origin licensing and replication stress. Collectively, our data reveal the tumour-promoting ability of p21 through deregulation of DNA replication licensing machinery-an unorthodox role to be considered in cancer treatment, since p21 responds to various stimuli including some chemotherapy drugs.",
keywords = "Journal Article",
author = "Panagiotis Galanos and Konstantinos Vougas and David Walter and Alexander Polyzos and Apolinar Maya-Mendoza and Haagensen, {Emma J} and Antonis Kokkalis and Fani-Marlen Roumelioti and Sarantis Gagos and Maria Tzetis and Bego{\~n}a Canovas and Ana Igea and Ahuja, {Akshay K} and Ralph Zellweger and Sofia Havaki and Emanuel Kanavakis and Dimitris Kletsas and Roninson, {Igor B} and Garbis, {Spiros D} and Massimo Lopes and Angel Nebreda and Dimitris Thanos and Blow, {J Julian} and Paul Townsend and S{\o}rensen, {Claus Storgaard} and Jiri Bartek and Gorgoulis, {Vassilis G}",
year = "2016",
month = jul,
doi = "10.1038/ncb3378",
language = "English",
volume = "18",
pages = "777--89",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "nature publishing group",
number = "7",

}

RIS

TY - JOUR

T1 - Chronic p53-independent p21 expression causes genomic instability by deregulating replication licensing

AU - Galanos, Panagiotis

AU - Vougas, Konstantinos

AU - Walter, David

AU - Polyzos, Alexander

AU - Maya-Mendoza, Apolinar

AU - Haagensen, Emma J

AU - Kokkalis, Antonis

AU - Roumelioti, Fani-Marlen

AU - Gagos, Sarantis

AU - Tzetis, Maria

AU - Canovas, Begoña

AU - Igea, Ana

AU - Ahuja, Akshay K

AU - Zellweger, Ralph

AU - Havaki, Sofia

AU - Kanavakis, Emanuel

AU - Kletsas, Dimitris

AU - Roninson, Igor B

AU - Garbis, Spiros D

AU - Lopes, Massimo

AU - Nebreda, Angel

AU - Thanos, Dimitris

AU - Blow, J Julian

AU - Townsend, Paul

AU - Sørensen, Claus Storgaard

AU - Bartek, Jiri

AU - Gorgoulis, Vassilis G

PY - 2016/7

Y1 - 2016/7

N2 - The cyclin-dependent kinase inhibitor p21(WAF1/CIP1) (p21) is a cell-cycle checkpoint effector and inducer of senescence, regulated by p53. Yet, evidence suggests that p21 could also be oncogenic, through a mechanism that has so far remained obscure. We report that a subset of atypical cancerous cells strongly expressing p21 showed proliferation features. This occurred predominantly in p53-mutant human cancers, suggesting p53-independent upregulation of p21 selectively in more aggressive tumour cells. Multifaceted phenotypic and genomic analyses of p21-inducible, p53-null, cancerous and near-normal cellular models showed that after an initial senescence-like phase, a subpopulation of p21-expressing proliferating cells emerged, featuring increased genomic instability, aggressiveness and chemoresistance. Mechanistically, sustained p21 accumulation inhibited mainly the CRL4-CDT2 ubiquitin ligase, leading to deregulated origin licensing and replication stress. Collectively, our data reveal the tumour-promoting ability of p21 through deregulation of DNA replication licensing machinery-an unorthodox role to be considered in cancer treatment, since p21 responds to various stimuli including some chemotherapy drugs.

AB - The cyclin-dependent kinase inhibitor p21(WAF1/CIP1) (p21) is a cell-cycle checkpoint effector and inducer of senescence, regulated by p53. Yet, evidence suggests that p21 could also be oncogenic, through a mechanism that has so far remained obscure. We report that a subset of atypical cancerous cells strongly expressing p21 showed proliferation features. This occurred predominantly in p53-mutant human cancers, suggesting p53-independent upregulation of p21 selectively in more aggressive tumour cells. Multifaceted phenotypic and genomic analyses of p21-inducible, p53-null, cancerous and near-normal cellular models showed that after an initial senescence-like phase, a subpopulation of p21-expressing proliferating cells emerged, featuring increased genomic instability, aggressiveness and chemoresistance. Mechanistically, sustained p21 accumulation inhibited mainly the CRL4-CDT2 ubiquitin ligase, leading to deregulated origin licensing and replication stress. Collectively, our data reveal the tumour-promoting ability of p21 through deregulation of DNA replication licensing machinery-an unorthodox role to be considered in cancer treatment, since p21 responds to various stimuli including some chemotherapy drugs.

KW - Journal Article

U2 - 10.1038/ncb3378

DO - 10.1038/ncb3378

M3 - Journal article

C2 - 27323328

VL - 18

SP - 777

EP - 789

JO - Nature Cell Biology

JF - Nature Cell Biology

SN - 1465-7392

IS - 7

ER -

ID: 165716928