Characterizing the host coral proteome of Platygyra carnosa using suspension trapping (S-trap)
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Stony corals form the foundation of coral reefs, which are of prominent ecological and economic significance. A robust workflow for investigating the coral proteome is essential in understanding coral biology. Here we investigated different preparative workflows and characterized the proteome of Platygyra carnosa, a common stony coral of the South China Sea. We found that a combination of bead homogenization with suspension trapping (S-Trap) preparation could yield more than 2700 proteins from coral samples. Annotation using a P. carnosa transcriptome database revealed that the majority of proteins were from the coral host cells (2140, 212, and 427 proteins from host coral, dinoflagellate, and other compartments, respectively). Label-free quantification and functional annotations indicated that a high proportion were involved in protein and redox homeostasis. Furthermore, the S-Trap method achieved good reproducibility in quantitative analysis. Although yielding a low symbiont:host ratio, the method is efficient in characterizing the coral host proteomic landscape, which provides a foundation to explore the molecular basis of the responses of coral host tissues to environmental stressors.
Originalsprog | Engelsk |
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Tidsskrift | Journal of Proteome Research |
Vol/bind | 20 |
Udgave nummer | 3 |
Sider (fra-til) | 1783-1791 |
Antal sider | 9 |
ISSN | 1535-3893 |
DOI | |
Status | Udgivet - 2021 |
Eksternt udgivet | Ja |
ID: 379642997