Characterization of follicles in girls and young women with Turner syndrome who underwent ovarian tissue cryopreservation
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Characterization of follicles in girls and young women with Turner syndrome who underwent ovarian tissue cryopreservation. / Mamsen, Linn Salto; Charkiewicz, Karol; Anderson, Richard A; Telfer, Evelyn E; McLaughlin, Marie; Kelsey, Thomas W; Kristensen, Stine G; Gook, Debra A; Ernst, Erik; Andersen, Claus Yding.
I: Fertility and Sterility, Bind 111, Nr. 6, 2019, s. 1217-1225.e3.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Characterization of follicles in girls and young women with Turner syndrome who underwent ovarian tissue cryopreservation
AU - Mamsen, Linn Salto
AU - Charkiewicz, Karol
AU - Anderson, Richard A
AU - Telfer, Evelyn E
AU - McLaughlin, Marie
AU - Kelsey, Thomas W
AU - Kristensen, Stine G
AU - Gook, Debra A
AU - Ernst, Erik
AU - Andersen, Claus Yding
PY - 2019
Y1 - 2019
N2 - OBJECTIVE: To characterize ovarian follicles of girls and young women with Turner syndrome (TS) who underwent ovarian tissue cryopreservation (OTC).DESIGN: Retrospective case-control study.SETTING: University hospital.PATIENT(S): Fifteen girls and young women with TS aged 5-22 years at OTC were included, together with 42 control girls and young women aged 1-25 years who underwent OTC because of cancer.INTERVENTION(S): None.MAIN OUTCOME MEASURE(S): Follicle density (follicles/mm3), morphology, and health were assessed in ovarian cortex biopsies from TS patients and compared with controls. Hormone concentrations were measured in serum and follicle fluids. Immature cumulus oocyte complexes were obtained and matured in vitro.RESULT(S): Follicles were found in 60% of the biopsies (9 of 15) from TS ovaries. In 78% of the ovaries (7 of 9) with follicles, the follicle density was within the 95% confidence interval of the control group. There was a high rate of abnormal follicle morphology. Six follicle-specific proteins were expressed similarly in TS and control ovaries. However, apoptosis and zona pellucida protein expression were found to be abnormal in TS. Turner syndrome follicle fluid from small antral follicles had lower concentrations of estrogen and testosterone and higher concentrations of antimüllerian hormone than controls. Thirty-one cumulus oocyte complexes were collected from one patient and cultured for 48 hours in vitro, resulting in five metaphase II oocytes (maturation rate 16%, degeneration rate 19%).CONCLUSION(S): The benefits of OTC may be limited to a highly selected group of TS mosaic patients in whom a sizeable pool of normal follicles is present at OTC.
AB - OBJECTIVE: To characterize ovarian follicles of girls and young women with Turner syndrome (TS) who underwent ovarian tissue cryopreservation (OTC).DESIGN: Retrospective case-control study.SETTING: University hospital.PATIENT(S): Fifteen girls and young women with TS aged 5-22 years at OTC were included, together with 42 control girls and young women aged 1-25 years who underwent OTC because of cancer.INTERVENTION(S): None.MAIN OUTCOME MEASURE(S): Follicle density (follicles/mm3), morphology, and health were assessed in ovarian cortex biopsies from TS patients and compared with controls. Hormone concentrations were measured in serum and follicle fluids. Immature cumulus oocyte complexes were obtained and matured in vitro.RESULT(S): Follicles were found in 60% of the biopsies (9 of 15) from TS ovaries. In 78% of the ovaries (7 of 9) with follicles, the follicle density was within the 95% confidence interval of the control group. There was a high rate of abnormal follicle morphology. Six follicle-specific proteins were expressed similarly in TS and control ovaries. However, apoptosis and zona pellucida protein expression were found to be abnormal in TS. Turner syndrome follicle fluid from small antral follicles had lower concentrations of estrogen and testosterone and higher concentrations of antimüllerian hormone than controls. Thirty-one cumulus oocyte complexes were collected from one patient and cultured for 48 hours in vitro, resulting in five metaphase II oocytes (maturation rate 16%, degeneration rate 19%).CONCLUSION(S): The benefits of OTC may be limited to a highly selected group of TS mosaic patients in whom a sizeable pool of normal follicles is present at OTC.
KW - Adolescent
KW - Adult
KW - Biopsy
KW - Child
KW - Child, Preschool
KW - Clinical Decision-Making
KW - Cryopreservation
KW - Female
KW - Fertility
KW - Fertility Preservation/methods
KW - Follicular Fluid/chemistry
KW - Genetic Predisposition to Disease
KW - Humans
KW - In Vitro Oocyte Maturation Techniques
KW - Infant
KW - Infertility, Female/etiology
KW - Ovarian Follicle/chemistry
KW - Ovary/chemistry
KW - Patient Selection
KW - Phenotype
KW - Predictive Value of Tests
KW - Reproductive Techniques, Assisted
KW - Retrospective Studies
KW - Turner Syndrome/complications
KW - Young Adult
U2 - 10.1016/j.fertnstert.2019.02.003
DO - 10.1016/j.fertnstert.2019.02.003
M3 - Journal article
C2 - 30922638
VL - 111
SP - 1217-1225.e3
JO - Sexuality, Reproduction and Menopause
JF - Sexuality, Reproduction and Menopause
SN - 1546-2501
IS - 6
ER -
ID: 241895919