Changing Smoking Behavior and Epigenetics: A Longitudinal Study of 4,432 Individuals From the General Population
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Changing Smoking Behavior and Epigenetics : A Longitudinal Study of 4,432 Individuals From the General Population. / Skov-Jeppesen, Sune Moeller; Kobylecki, Camilla Jannie; Jacobsen, Katja Kemp; Bojesen, Stig Egil.
I: Chest, Bind 163, Nr. 6, 2023, s. 1565-1575.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Changing Smoking Behavior and Epigenetics
T2 - A Longitudinal Study of 4,432 Individuals From the General Population
AU - Skov-Jeppesen, Sune Moeller
AU - Kobylecki, Camilla Jannie
AU - Jacobsen, Katja Kemp
AU - Bojesen, Stig Egil
N1 - Publisher Copyright: © 2023 The Author(s)
PY - 2023
Y1 - 2023
N2 - Background: Hypomethylation of the aryl hydrocarbon receptor repressor (AHRR) gene indicates long-term smoking exposure and might therefore be a monitor for smoking-induced disease risk. However, studies of individual longitudinal changes in AHRR methylation are sparse. Research Question: How does the recovery of AHRR methylation depend on change in smoking behaviors and demographic variables? Study Design and Methods: This study included 4,432 individuals from the Copenhagen City Heart Study, with baseline and follow-up blood samples and smoking information collected approximately 10 years apart. AHRR methylation at the cg05575921 site was measured in bisulfite-treated leukocyte DNA. Four smoking groups were defined: participants who never smoked (Never-Never), participants who formerly smoked (Former-Former), participants who quit during the study period (Current-Former), and individuals who smoked at both baseline and follow-up (Current-Current). Methylation recovery was defined as the increase in AHRR methylation between baseline and follow-up examination. Results: Methylation recovery was highest among participants who quit, with a median methylation recovery of 5.58% (interquartile range, 1.79; 9.15) vs 1.64% (interquartile range, –1.88; 4.96) in the Current-Current group (P < .0001). In individuals who quit smoking, older age was associated with lower methylation recovery (P < .0001). In participants who quit aged > 65 years, methylation recovery was 5.9% at 5.6 years after quitting; methylation recovery was 8.5% after 2.8 years for participants who quit aged < 55 years. Interpretation: AHRR methylation recovered after individuals quit smoking, and recovery was more pronounced and occurred faster in younger compared with older interim quitters.
AB - Background: Hypomethylation of the aryl hydrocarbon receptor repressor (AHRR) gene indicates long-term smoking exposure and might therefore be a monitor for smoking-induced disease risk. However, studies of individual longitudinal changes in AHRR methylation are sparse. Research Question: How does the recovery of AHRR methylation depend on change in smoking behaviors and demographic variables? Study Design and Methods: This study included 4,432 individuals from the Copenhagen City Heart Study, with baseline and follow-up blood samples and smoking information collected approximately 10 years apart. AHRR methylation at the cg05575921 site was measured in bisulfite-treated leukocyte DNA. Four smoking groups were defined: participants who never smoked (Never-Never), participants who formerly smoked (Former-Former), participants who quit during the study period (Current-Former), and individuals who smoked at both baseline and follow-up (Current-Current). Methylation recovery was defined as the increase in AHRR methylation between baseline and follow-up examination. Results: Methylation recovery was highest among participants who quit, with a median methylation recovery of 5.58% (interquartile range, 1.79; 9.15) vs 1.64% (interquartile range, –1.88; 4.96) in the Current-Current group (P < .0001). In individuals who quit smoking, older age was associated with lower methylation recovery (P < .0001). In participants who quit aged > 65 years, methylation recovery was 5.9% at 5.6 years after quitting; methylation recovery was 8.5% after 2.8 years for participants who quit aged < 55 years. Interpretation: AHRR methylation recovered after individuals quit smoking, and recovery was more pronounced and occurred faster in younger compared with older interim quitters.
KW - aryl hydrocarbon receptor repressor
KW - DNA methylation
KW - longitudinal study
KW - lung cancer
KW - smoking
U2 - 10.1016/j.chest.2022.12.036
DO - 10.1016/j.chest.2022.12.036
M3 - Journal article
C2 - 36621758
AN - SCOPUS:85159892058
VL - 163
SP - 1565
EP - 1575
JO - Chest
JF - Chest
SN - 0012-3692
IS - 6
ER -
ID: 362895307