Centrifugally driven microfluidic disc for detection of chromosomal translocations
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Centrifugally driven microfluidic disc for detection of chromosomal translocations. / Brøgger, Anna Line; Kwasny, Dorota; Bosco, Filippo; Silahtaroglu, Asli; Tümer, Zeynep; Boisen, Anja; Svendsen, Winnie Edith.
I: Lab On a Chip, Bind 12, Nr. 22, 21.11.2012, s. 4628-4634.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Centrifugally driven microfluidic disc for detection of chromosomal translocations
AU - Brøgger, Anna Line
AU - Kwasny, Dorota
AU - Bosco, Filippo
AU - Silahtaroglu, Asli
AU - Tümer, Zeynep
AU - Boisen, Anja
AU - Svendsen, Winnie Edith
PY - 2012/11/21
Y1 - 2012/11/21
N2 - Chromosome translocations are a common cause of congenital disorders and cancer. Current detection methods require use of expensive and highly specialized techniques to identify the chromosome regions involved in a translocation. There is a need for rapid yet specific detection for diagnosis and prognosis of patients. In this work we demonstrate a novel, centrifugally-driven microfluidic system for controlled manipulation of oligonucleotides and subsequent detection of chromosomal translocations. The device is fabricated in the form of a disc with capillary burst microvalves employed to control the fluid flow. The microvalves in series are designed to enable fluid movement from the center towards the periphery of the disc to handle DNA sequences representing translocation between chromosome 3 and 9. The translocation detection is performed in two hybridization steps in separate sorting and detection chambers. The burst frequencies of the two capillary burst microvalves are separated by 180 rpm enabling precise control of hybridization in each of the chambers. The DNA probes targeting a translocation are immobilized directly on PMMA by a UV-activated procedure, which is compatible with the disc fabrication method. The device performance was validated by successful specific hybridization of the translocation derivatives in the sorting and detection chambers.
AB - Chromosome translocations are a common cause of congenital disorders and cancer. Current detection methods require use of expensive and highly specialized techniques to identify the chromosome regions involved in a translocation. There is a need for rapid yet specific detection for diagnosis and prognosis of patients. In this work we demonstrate a novel, centrifugally-driven microfluidic system for controlled manipulation of oligonucleotides and subsequent detection of chromosomal translocations. The device is fabricated in the form of a disc with capillary burst microvalves employed to control the fluid flow. The microvalves in series are designed to enable fluid movement from the center towards the periphery of the disc to handle DNA sequences representing translocation between chromosome 3 and 9. The translocation detection is performed in two hybridization steps in separate sorting and detection chambers. The burst frequencies of the two capillary burst microvalves are separated by 180 rpm enabling precise control of hybridization in each of the chambers. The DNA probes targeting a translocation are immobilized directly on PMMA by a UV-activated procedure, which is compatible with the disc fabrication method. The device performance was validated by successful specific hybridization of the translocation derivatives in the sorting and detection chambers.
U2 - 10.1039/c2lc40554g
DO - 10.1039/c2lc40554g
M3 - Journal article
C2 - 22911443
VL - 12
SP - 4628
EP - 4634
JO - Lab on a Chip
JF - Lab on a Chip
SN - 1473-0197
IS - 22
ER -
ID: 40840799