Cell-type-specific H+-ATPase activity in root tissues enables K+ retention and mediates acclimation of barley (Hordeum vulgare) to salinity stress
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Cell-type-specific H+-ATPase activity in root tissues enables K+ retention and mediates acclimation of barley (Hordeum vulgare) to salinity stress. / Shabala, Lana; Zhang, Jingyi; Pottosin, Igor; Bose, Jayakumar; Zhu, Min; Fuglsang, Anja Thoe; Velarde-Buendia, Ana; Massart, Amandine; Hill, Camilla Beate; Roessner, Ute; Bacic, Antony; Wu, Honghong; Azzarello, Elisa; Pandolfi, Camilla; Zhou, Meixue; Poschenrieder, Charlotte; Mancuso, Stefano; Shabala, Sergey.
I: Plant Physiology, Bind 172, Nr. 4, 2016, s. 2445-2458.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Cell-type-specific H+-ATPase activity in root tissues enables K+ retention and mediates acclimation of barley (Hordeum vulgare) to salinity stress
AU - Shabala, Lana
AU - Zhang, Jingyi
AU - Pottosin, Igor
AU - Bose, Jayakumar
AU - Zhu, Min
AU - Fuglsang, Anja Thoe
AU - Velarde-Buendia, Ana
AU - Massart, Amandine
AU - Hill, Camilla Beate
AU - Roessner, Ute
AU - Bacic, Antony
AU - Wu, Honghong
AU - Azzarello, Elisa
AU - Pandolfi, Camilla
AU - Zhou, Meixue
AU - Poschenrieder, Charlotte
AU - Mancuso, Stefano
AU - Shabala, Sergey
N1 - © 2016 American Society of Plant Biologists. All Rights Reserved.
PY - 2016
Y1 - 2016
N2 - While the importance of cell type specificity in plant adaptive responses is widely accepted, only a limited number of studies have addressed this issue at the functional level. We have combined electrophysiological, imaging, and biochemical techniques to reveal the physiological mechanisms conferring higher sensitivity of apical root cells to salinity in barley (Hordeum vulgare). We show that salinity application to the root apex arrests root growth in a highly tissue- and treatment-specific manner. Although salinity-induced transient net Na(+) uptake was about 4-fold higher in the root apex compared with the mature zone, mature root cells accumulated more cytosolic and vacuolar Na(+), suggesting that the higher sensitivity of apical cells to salt is not related to either enhanced Na(+) exclusion or sequestration inside the root. Rather, the above differential sensitivity between the two zones originates from a 10-fold difference in K(+) efflux between the mature zone and the apical region (much poorer in the root apex) of the root. Major factors contributing to this poor K(+) retention ability are (1) an intrinsically lower H(+)-ATPase activity in the root apex, (2) greater salt-induced membrane depolarization, and (3) a higher reactive oxygen species production under NaCl and a larger density of reactive oxygen species-activated cation currents in the apex. Salinity treatment increased (2- to 5-fold) the content of 10 (out of 25 detected) amino acids in the root apex but not in the mature zone and changed the organic acid and sugar contents. The causal link between the observed changes in the root metabolic profile and the regulation of transporter activity is discussed.
AB - While the importance of cell type specificity in plant adaptive responses is widely accepted, only a limited number of studies have addressed this issue at the functional level. We have combined electrophysiological, imaging, and biochemical techniques to reveal the physiological mechanisms conferring higher sensitivity of apical root cells to salinity in barley (Hordeum vulgare). We show that salinity application to the root apex arrests root growth in a highly tissue- and treatment-specific manner. Although salinity-induced transient net Na(+) uptake was about 4-fold higher in the root apex compared with the mature zone, mature root cells accumulated more cytosolic and vacuolar Na(+), suggesting that the higher sensitivity of apical cells to salt is not related to either enhanced Na(+) exclusion or sequestration inside the root. Rather, the above differential sensitivity between the two zones originates from a 10-fold difference in K(+) efflux between the mature zone and the apical region (much poorer in the root apex) of the root. Major factors contributing to this poor K(+) retention ability are (1) an intrinsically lower H(+)-ATPase activity in the root apex, (2) greater salt-induced membrane depolarization, and (3) a higher reactive oxygen species production under NaCl and a larger density of reactive oxygen species-activated cation currents in the apex. Salinity treatment increased (2- to 5-fold) the content of 10 (out of 25 detected) amino acids in the root apex but not in the mature zone and changed the organic acid and sugar contents. The causal link between the observed changes in the root metabolic profile and the regulation of transporter activity is discussed.
U2 - 10.1104/pp.16.01347
DO - 10.1104/pp.16.01347
M3 - Journal article
C2 - 27770060
VL - 172
SP - 2445
EP - 2458
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 4
ER -
ID: 169991253