Cell-Based Glycan Arrays — A Practical Guide to Dissect the Human Glycome
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Cell-Based Glycan Arrays — A Practical Guide to Dissect the Human Glycome. / Büll, Christian; Joshi, Hiren Jitendra; Clausen, Henrik; Narimatsu, Yoshiki.
I: STAR Protocols, Bind 1, Nr. 1, 100017, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Cell-Based Glycan Arrays — A Practical Guide to Dissect the Human Glycome
AU - Büll, Christian
AU - Joshi, Hiren Jitendra
AU - Clausen, Henrik
AU - Narimatsu, Yoshiki
PY - 2020
Y1 - 2020
N2 - Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).
AB - Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).
U2 - 10.1016/j.xpro.2020.100017
DO - 10.1016/j.xpro.2020.100017
M3 - Journal article
C2 - 33111073
VL - 1
JO - STAR Protocols
JF - STAR Protocols
IS - 1
M1 - 100017
ER -
ID: 237909164