Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein
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Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein. / Bang-Christensen, Sara R; Pedersen, Rasmus S; Pereira, Marina A; Clausen, Thomas M; Løppke, Caroline; Sand, Nicolai T; Ahrens, Theresa D; Jørgensen, Amalie M; Lim, Yi Chieh; Goksøyr, Louise; Choudhary, Swati; Gustavsson, Tobias; Dagil, Robert; Daugaard, Mads; Sander, Adam F; Torp, Mathias H; Søgaard, Max; Theander, Thor G; Østrup, Olga; Lassen, Ulrik; Hamerlik, Petra; Salanti, Ali; Agerbæk, Mette Ø.
I: Cells, Bind 8, Nr. 9, 998, 2019.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein
AU - Bang-Christensen, Sara R
AU - Pedersen, Rasmus S
AU - Pereira, Marina A
AU - Clausen, Thomas M
AU - Løppke, Caroline
AU - Sand, Nicolai T
AU - Ahrens, Theresa D
AU - Jørgensen, Amalie M
AU - Lim, Yi Chieh
AU - Goksøyr, Louise
AU - Choudhary, Swati
AU - Gustavsson, Tobias
AU - Dagil, Robert
AU - Daugaard, Mads
AU - Sander, Adam F
AU - Torp, Mathias H
AU - Søgaard, Max
AU - Theander, Thor G
AU - Østrup, Olga
AU - Lassen, Ulrik
AU - Hamerlik, Petra
AU - Salanti, Ali
AU - Agerbæk, Mette Ø
PY - 2019
Y1 - 2019
N2 - Diffuse gliomas are the most common primary malignant brain tumor. Although extracranial metastases are rarely observed, recent studies have shown the presence of circulating tumor cells (CTCs) in the blood of glioma patients, confirming that a subset of tumor cells are capable of entering the circulation. The isolation and characterization of CTCs could provide a non-invasive method for repeated analysis of the mutational and phenotypic state of the tumor during the course of disease. However, the efficient detection of glioma CTCs has proven to be challenging due to the lack of consistently expressed tumor markers and high inter- and intra-tumor heterogeneity. Thus, for this field to progress, an omnipresent but specific marker of glioma CTCs is required. In this article, we demonstrate how the recombinant malaria VAR2CSA protein (rVAR2) can be used for the capture and detection of glioma cell lines that are spiked into blood through binding to a cancer-specific oncofetal chondroitin sulfate (ofCS). When using rVAR2 pull-down from glioma cells, we identified a panel of proteoglycans, known to be essential for glioma progression. Finally, the clinical feasibility of this work is supported by the rVAR2-based isolation and detection of CTCs from glioma patient blood samples, which highlights ofCS as a potential clinical target for CTC isolation.
AB - Diffuse gliomas are the most common primary malignant brain tumor. Although extracranial metastases are rarely observed, recent studies have shown the presence of circulating tumor cells (CTCs) in the blood of glioma patients, confirming that a subset of tumor cells are capable of entering the circulation. The isolation and characterization of CTCs could provide a non-invasive method for repeated analysis of the mutational and phenotypic state of the tumor during the course of disease. However, the efficient detection of glioma CTCs has proven to be challenging due to the lack of consistently expressed tumor markers and high inter- and intra-tumor heterogeneity. Thus, for this field to progress, an omnipresent but specific marker of glioma CTCs is required. In this article, we demonstrate how the recombinant malaria VAR2CSA protein (rVAR2) can be used for the capture and detection of glioma cell lines that are spiked into blood through binding to a cancer-specific oncofetal chondroitin sulfate (ofCS). When using rVAR2 pull-down from glioma cells, we identified a panel of proteoglycans, known to be essential for glioma progression. Finally, the clinical feasibility of this work is supported by the rVAR2-based isolation and detection of CTCs from glioma patient blood samples, which highlights ofCS as a potential clinical target for CTC isolation.
U2 - 10.3390/cells8090998
DO - 10.3390/cells8090998
M3 - Journal article
C2 - 31466397
VL - 8
JO - Cells
JF - Cells
SN - 2073-4409
IS - 9
M1 - 998
ER -
ID: 238529867