Bruch's membrane allows unhindered passage of up to 2 μm latex beads in an in vivo porcine model
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Bruch's membrane allows unhindered passage of up to 2 μm latex beads in an in vivo porcine model. / Sørensen, Nina Buus; Christiansen, Anders Tolstrup; Kjær, Troels Wesenberg; Klemp, Kristian; la Cour, Morten; Heegaard, Steffen; Kiilgaard, Jens Folke.
I: Experimental Eye Research, Bind 180, 2019, s. 1-7.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Bruch's membrane allows unhindered passage of up to 2 μm latex beads in an in vivo porcine model
AU - Sørensen, Nina Buus
AU - Christiansen, Anders Tolstrup
AU - Kjær, Troels Wesenberg
AU - Klemp, Kristian
AU - la Cour, Morten
AU - Heegaard, Steffen
AU - Kiilgaard, Jens Folke
N1 - Copyright © 2018. Published by Elsevier Ltd.
PY - 2019
Y1 - 2019
N2 - PURPOSE: It has been proposed that changes in the permeability of Bruch's membrane play a role in the pathogenesis of age-related macular degeneration (AMD). This paper investigates, in an in vivo porcine model, the migration of fluorescent latex beads across the Bruch's membrane after subretinal injection.METHODS: Forty-one healthy eyes of 33 three-month-old domestic pigs received a subretinal injection of 0.5, 1.0, 2.0, or 4.0 μm fluorescent latex beads. Between three hours and five weeks after injection evaluations were performed with fundus photographs and histology. Fluorescent beads were identified in unstained histologic sections using the rhodamine filter with the light microscope.RESULTS: The fluorescent latex beads relocated from the subretinal space. Intact beads up to 2.0 μm were found in the choroid, sclera, and extrascleral space. The smaller beads were also found inside choroidal and extrascleral blood vessels. In contrast, the larger beads of 4.0 μm did not pass the Bruch's membrane.CONCLUSION: Subretinally implanted beads up to 2.0 μm pass the Bruch's membrane intact and cross the blood-ocular barrier. The intact beads are found in the choroid, sclera and inside blood vessels. The results give reason to consider the role of subretinal clearance and passage of Bruch's membrane in the development of AMD.
AB - PURPOSE: It has been proposed that changes in the permeability of Bruch's membrane play a role in the pathogenesis of age-related macular degeneration (AMD). This paper investigates, in an in vivo porcine model, the migration of fluorescent latex beads across the Bruch's membrane after subretinal injection.METHODS: Forty-one healthy eyes of 33 three-month-old domestic pigs received a subretinal injection of 0.5, 1.0, 2.0, or 4.0 μm fluorescent latex beads. Between three hours and five weeks after injection evaluations were performed with fundus photographs and histology. Fluorescent beads were identified in unstained histologic sections using the rhodamine filter with the light microscope.RESULTS: The fluorescent latex beads relocated from the subretinal space. Intact beads up to 2.0 μm were found in the choroid, sclera, and extrascleral space. The smaller beads were also found inside choroidal and extrascleral blood vessels. In contrast, the larger beads of 4.0 μm did not pass the Bruch's membrane.CONCLUSION: Subretinally implanted beads up to 2.0 μm pass the Bruch's membrane intact and cross the blood-ocular barrier. The intact beads are found in the choroid, sclera and inside blood vessels. The results give reason to consider the role of subretinal clearance and passage of Bruch's membrane in the development of AMD.
U2 - 10.1016/j.exer.2018.11.019
DO - 10.1016/j.exer.2018.11.019
M3 - Journal article
C2 - 30468719
VL - 180
SP - 1
EP - 7
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
ER -
ID: 209391586