Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics
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Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics. / Stangegaard, Michael; Tomas, Carmen; Hansen, Anders J.; Frank-Hansen, Rune; Børsting, Claus; Morling, Niels.
I: JALA - Journal of the Association for Laboratory Automation, Bind 13, Nr. 5, 2008, s. 297-303.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics
AU - Stangegaard, Michael
AU - Tomas, Carmen
AU - Hansen, Anders J.
AU - Frank-Hansen, Rune
AU - Børsting, Claus
AU - Morling, Niels
PY - 2008
Y1 - 2008
N2 - Single nucleotide polymorphism genotyping provides a supplement for conventional short tandem repeats-based kits currently used for human identification. GenPlex (Applied Biosystems (AB), Foster City, CA) is an SNP-genotyping kit based on a multiplex of 48 informative, autosomal SNPs from the SNPforID Consortium. Our objective was to setup, implement, and validate a small and affordable automated liquid-handling robot for forensic casework samples (buccal swaps on FTA-paper and Qiagen purified blood). The reaction scheme consisted of numerous steps and was cumbersome to perform consistently manually. Automation was accomplished with a Biomek-3000 (Beckmann Coulter) laboratory-automated workstation using five in-house-developed methods. All methods allowed the user to select the number of subsequent injections to the capillary electrophoresis instrument (ABI 3130xl, AB) enabling processing of both partial and full plates. A total of 286 samples were analyzed in duplicates with the GenPlex reaction using the Biomek-3000. The results were compared with those obtained from the same samples using the SNaPshot(AB) single-base extension system. Full concordance of the results was obtained in all but one sample. The results demonstrate that the Biomek-3000 can perform a series of complex reactions leading to highly consistent forensic genetic SNP-typing results Udgivelsesdato: 2008/10
AB - Single nucleotide polymorphism genotyping provides a supplement for conventional short tandem repeats-based kits currently used for human identification. GenPlex (Applied Biosystems (AB), Foster City, CA) is an SNP-genotyping kit based on a multiplex of 48 informative, autosomal SNPs from the SNPforID Consortium. Our objective was to setup, implement, and validate a small and affordable automated liquid-handling robot for forensic casework samples (buccal swaps on FTA-paper and Qiagen purified blood). The reaction scheme consisted of numerous steps and was cumbersome to perform consistently manually. Automation was accomplished with a Biomek-3000 (Beckmann Coulter) laboratory-automated workstation using five in-house-developed methods. All methods allowed the user to select the number of subsequent injections to the capillary electrophoresis instrument (ABI 3130xl, AB) enabling processing of both partial and full plates. A total of 286 samples were analyzed in duplicates with the GenPlex reaction using the Biomek-3000. The results were compared with those obtained from the same samples using the SNaPshot(AB) single-base extension system. Full concordance of the results was obtained in all but one sample. The results demonstrate that the Biomek-3000 can perform a series of complex reactions leading to highly consistent forensic genetic SNP-typing results Udgivelsesdato: 2008/10
U2 - doi:10.1016/j.jala.2008.05.006
DO - doi:10.1016/j.jala.2008.05.006
M3 - Journal article
VL - 13
SP - 297
EP - 303
JO - SLAS TECHNOLOGY: Translating Life Sciences Innovation
JF - SLAS TECHNOLOGY: Translating Life Sciences Innovation
SN - 2211-0682
IS - 5
ER -
ID: 14440048