Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa
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BACKGROUND: Chronic non-healing or recurrent inflammatory lesions, reminiscent of infection but recalcitrant to antibiotic therapy generally characterize biofilm driven-diseases. Chronic lesions of Hidradenitis Suppurativa (HS) exhibit several aspects, which are compatible with well-known biofilm infections.
OBJECTIVE: To determine and quantify the potential presence of bacterial aggregates in chronic HS lesions.
METHODS: In 42 consecutive HS patients suffering from chronic lesions, biopsies were obtained from lesional as well as from perilesional skin. Samples were investigated using Peptide Nucleic Acid (PNA) - Fluorescence in situ Hybridization (FISH) in combination with Confocal Laser Scanning Microscopy (CLSM). In addition, corresponding histopathological analysis in hematoxylin and eosin slides were performed.
RESULTS: Biofilms were seen in 67% of the samples of chronic lesions and in 75% of the perilesional samples. The mean diameter of aggregates in lesional skin was significantly greater than in perilesional skin (p=0.01). Biofilms exceeding 50 μm in diameter were found in 42% of lesional samples and only in only 5% of the perilesional samples (p=0.009). The majority of the large biofilms (aggregates > 50 μm in diameter) were situated in sinus tracts (63%) or in the infundibulum (37%). The majority of the sinus tract samples (73%) contained active bacterial cells, which were associated with inflammation.
CONCLUSION: This study suggests that biofilm is associated with inflammation of chronic HS lesions. The aggregates most likely occur as a secondary event, possibly due to predisposing local anatomical changes such as sinus tracts (tunnels), keratinous detritus and dilated hair follicles. This article is protected by copyright. All rights reserved.
Originalsprog | Engelsk |
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Tidsskrift | British Journal of Dermatology |
Vol/bind | 176 |
Udgave nummer | 4 |
Sider (fra-til) | 993–1000 |
ISSN | 0007-0963 |
DOI | |
Status | Udgivet - 2017 |
ID: 170702236