Assessment of biomarkers in pediatric atopic dermatitis by tape strips and skin biopsies
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Assessment of biomarkers in pediatric atopic dermatitis by tape strips and skin biopsies. / Andersson, Anna Maria; Sølberg, Julie; Koch, Anders; Skov, Lone; Jakasa, Ivone; Kezic, Sanja; Thyssen, Jacob Pontoppidan.
I: Allergy: European Journal of Allergy and Clinical Immunology, Bind 77, Nr. 5, 2022, s. 1499-1509.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Assessment of biomarkers in pediatric atopic dermatitis by tape strips and skin biopsies
AU - Andersson, Anna Maria
AU - Sølberg, Julie
AU - Koch, Anders
AU - Skov, Lone
AU - Jakasa, Ivone
AU - Kezic, Sanja
AU - Thyssen, Jacob Pontoppidan
N1 - Funding information: This study was supported by a grant from LEO Pharma Research Foundation.
PY - 2022
Y1 - 2022
N2 - Background: The cytokine profile of atopic dermatitis (AD) depends on age, ethnicity, and disease severity. This study examined biomarkers in children with AD collected by tape strips and skin biopsies, and examined whether the levels differed with filaggrin genotype, disease severity, and food allergy. Methods: Twenty-five children aged 2–14 years with AD were clinically examined. Skin biopsies were collected from lesional skin and tape strips were collected from lesional and non-lesional skin. We analyzed natural moisturizing factor (NMF) and 17 immune markers represented by mRNA levels in skin biopsies and protein levels in tape strips. Common filaggrin gene mutations were examined in all children. Results: The cytokine profile in lesional skin was dominated by a T helper (Th) 2 response in skin biopsies, and by a general increase in innate inflammation markers (interleukin (IL)-1α, IL-1β, IL-8, IL-18) along with TARC and CTACK in tape strips. The levels of TARC, CTACK, IL-8, IL-18 showed significant correlation with AD severity in both lesional and non-lesional tape stripped skin, while no significant correlations were observed in skin biopsy data. In tape strips from lesional and non-lesional skin, the levels of NMF and selected cytokines differed significantly between children with and without FLG mutations and food allergy. Conclusion: Sampling of the stratum corneum with non-invasive tape strips can be used to identify biomarkers that are associated with disease severity, food allergy and FLG mutations. Skin biopsies showed robust Th2 signature but was inferior for association analysis regarding severity.
AB - Background: The cytokine profile of atopic dermatitis (AD) depends on age, ethnicity, and disease severity. This study examined biomarkers in children with AD collected by tape strips and skin biopsies, and examined whether the levels differed with filaggrin genotype, disease severity, and food allergy. Methods: Twenty-five children aged 2–14 years with AD were clinically examined. Skin biopsies were collected from lesional skin and tape strips were collected from lesional and non-lesional skin. We analyzed natural moisturizing factor (NMF) and 17 immune markers represented by mRNA levels in skin biopsies and protein levels in tape strips. Common filaggrin gene mutations were examined in all children. Results: The cytokine profile in lesional skin was dominated by a T helper (Th) 2 response in skin biopsies, and by a general increase in innate inflammation markers (interleukin (IL)-1α, IL-1β, IL-8, IL-18) along with TARC and CTACK in tape strips. The levels of TARC, CTACK, IL-8, IL-18 showed significant correlation with AD severity in both lesional and non-lesional tape stripped skin, while no significant correlations were observed in skin biopsy data. In tape strips from lesional and non-lesional skin, the levels of NMF and selected cytokines differed significantly between children with and without FLG mutations and food allergy. Conclusion: Sampling of the stratum corneum with non-invasive tape strips can be used to identify biomarkers that are associated with disease severity, food allergy and FLG mutations. Skin biopsies showed robust Th2 signature but was inferior for association analysis regarding severity.
KW - atopic dermatitis
KW - biomarker
KW - filaggrin
KW - immunotype
KW - pediatric
U2 - 10.1111/all.15153
DO - 10.1111/all.15153
M3 - Journal article
C2 - 34695223
AN - SCOPUS:85118535344
VL - 77
SP - 1499
EP - 1509
JO - Allergy: European Journal of Allergy and Clinical Immunology
JF - Allergy: European Journal of Allergy and Clinical Immunology
SN - 0105-4538
IS - 5
ER -
ID: 286994544