Artificial Tears, Preservatives and so what?
Publikation: Bidrag til tidsskrift › Konferenceabstrakt i tidsskrift › Forskning › fagfællebedømt
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Artificial Tears, Preservatives and so what? / Alnoor, Umalbaninn Mokdad Kazem; Nordqvist Graver Ahrensberg, Simone ; Freiberg, Josefine; Nagstrup, Anne Hedengran; Heegaard, Steffen; Kolko, Miriam.
I: Acta Ophthalmologica, Bind 102, Nr. S279, 2024.Publikation: Bidrag til tidsskrift › Konferenceabstrakt i tidsskrift › Forskning › fagfællebedømt
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T1 - Artificial Tears, Preservatives and so what?
AU - Alnoor, Umalbaninn Mokdad Kazem
AU - Nordqvist Graver Ahrensberg, Simone
AU - Freiberg, Josefine
AU - Nagstrup, Anne Hedengran
AU - Heegaard, Steffen
AU - Kolko, Miriam
N1 - Special Issue:Abstracts from the 2023 European Association for Vision and Eye Research Festival, 26‐28 October 2023, Valencia.
PY - 2024
Y1 - 2024
N2 - Aims/Purpose: To investigate the cell survival of human conjunctival goblet cells (CGs) when incubated with preserved and preservative-free (PF) artificial tears (AT) used against dry eye disease (DED). The following ATs were tested: 0.011% benzalkonium chloride (BAK)-preserved Viskøse® Øjendråber, 0.006% BAK-preserved Oftagel®, 0.005% BAK-preserved Oculac®, 0.001% PolyQuad™-preserved Systane® Ultra, 0.01% Cetrimide-preserved Artelac®, and PF ATs: Systane® Ultra, Oculac®, Oftagel®, and Thealoz® Duo.Methods: Primary cultures of human conjunctival CGs were cultivated from donor conjunctiva. After incubation with the above-mentioned ATs for 2 h, CG survival was analysed using a lactate dehydrogenase assay and calculated to a negative control.Results: AT preserved with 0.011% BAK reduced GC survival by 28% compared to negative control (p = 0.001). The remaining ATs did not significantly alter the cell survival.Conclusions: BAK-preserved ATs cause GC death in a concentration-dependent manner. Reduction of the GC density may contribute to development of DED. PF-ATs and PolyQuad- and Cetrimide-preserved ATs may thus be a better choice for treating DED than BAK preserved ATs, as the latter may add to the ocular surface disease.
AB - Aims/Purpose: To investigate the cell survival of human conjunctival goblet cells (CGs) when incubated with preserved and preservative-free (PF) artificial tears (AT) used against dry eye disease (DED). The following ATs were tested: 0.011% benzalkonium chloride (BAK)-preserved Viskøse® Øjendråber, 0.006% BAK-preserved Oftagel®, 0.005% BAK-preserved Oculac®, 0.001% PolyQuad™-preserved Systane® Ultra, 0.01% Cetrimide-preserved Artelac®, and PF ATs: Systane® Ultra, Oculac®, Oftagel®, and Thealoz® Duo.Methods: Primary cultures of human conjunctival CGs were cultivated from donor conjunctiva. After incubation with the above-mentioned ATs for 2 h, CG survival was analysed using a lactate dehydrogenase assay and calculated to a negative control.Results: AT preserved with 0.011% BAK reduced GC survival by 28% compared to negative control (p = 0.001). The remaining ATs did not significantly alter the cell survival.Conclusions: BAK-preserved ATs cause GC death in a concentration-dependent manner. Reduction of the GC density may contribute to development of DED. PF-ATs and PolyQuad- and Cetrimide-preserved ATs may thus be a better choice for treating DED than BAK preserved ATs, as the latter may add to the ocular surface disease.
U2 - 10.1111/aos.16012
DO - 10.1111/aos.16012
M3 - Conference abstract in journal
VL - 102
JO - Acta Ophthalmologica
JF - Acta Ophthalmologica
SN - 1755-375X
IS - S279
Y2 - 26 October 2023 through 28 October 2023
ER -
ID: 393781854