Apelin is a novel islet peptide
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Apelin is a novel islet peptide. / Ringström, Camilla; Nitert, Marloes Dekker; Bennet, Hedvig; Fex, Malin; Valet, Philippe; Rehfeld, Jens F.; Friis-Hansen, Lennart Jan; Wierup, Nils.
I: Regulatory Peptides, Bind 162, Nr. 1-3, 08.06.2010, s. 44-51.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Apelin is a novel islet peptide
AU - Ringström, Camilla
AU - Nitert, Marloes Dekker
AU - Bennet, Hedvig
AU - Fex, Malin
AU - Valet, Philippe
AU - Rehfeld, Jens F.
AU - Friis-Hansen, Lennart Jan
AU - Wierup, Nils
N1 - Copyright 2010 Elsevier B.V. All rights reserved.
PY - 2010/6/8
Y1 - 2010/6/8
N2 - Apelin, a recently discovered peptide with wide tissue distribution, regulates feeding behavior, improves glucose utilization, and inhibits insulin secretion. We examined whether apelin is expressed in human islets, as well as in normal and type 2 diabetic (T2D) animal islets. Further, we studied islet apelin regulation and the effect of apelin on insulin secretion. Apelin expression and regulation was examined in human and animal specimens using immunocytochemistry, in situ hybridization, and real-time PCR. Insulin secretion was studied in INS-1 (832/13) clonal beta cells. APJ-receptor expression was studied using real-time PCR. In human and murine islets apelin was predominantly expressed in beta cells and alpha cells; a subpopulation of the PP cells in human islets also harbored apelin. In porcine and feline islets apelin was mainly expressed in beta cells. APJ-receptor expression was detected in INS-1 (832/13) cells, and in human and mouse islets. A high dose (1microM) of apelin-36 caused a moderate increase in glucose-stimulated insulin secretion (30%; p
AB - Apelin, a recently discovered peptide with wide tissue distribution, regulates feeding behavior, improves glucose utilization, and inhibits insulin secretion. We examined whether apelin is expressed in human islets, as well as in normal and type 2 diabetic (T2D) animal islets. Further, we studied islet apelin regulation and the effect of apelin on insulin secretion. Apelin expression and regulation was examined in human and animal specimens using immunocytochemistry, in situ hybridization, and real-time PCR. Insulin secretion was studied in INS-1 (832/13) clonal beta cells. APJ-receptor expression was studied using real-time PCR. In human and murine islets apelin was predominantly expressed in beta cells and alpha cells; a subpopulation of the PP cells in human islets also harbored apelin. In porcine and feline islets apelin was mainly expressed in beta cells. APJ-receptor expression was detected in INS-1 (832/13) cells, and in human and mouse islets. A high dose (1microM) of apelin-36 caused a moderate increase in glucose-stimulated insulin secretion (30%; p
U2 - http://dx.doi.org/10.1016/j.regpep.2010.03.005
DO - http://dx.doi.org/10.1016/j.regpep.2010.03.005
M3 - Journal article
VL - 162
SP - 44
EP - 51
JO - Regulatory Peptides
JF - Regulatory Peptides
SN - 0167-0115
IS - 1-3
ER -
ID: 34179609