Anti-CRISPR Proteins in Archaea
Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
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Anti-CRISPR Proteins in Archaea. / Peng, Xu; Mayo-Muñoz, David; Bhoobalan-Chitty, Yuvaraj; Martínez-Álvarez, Laura.
I: Trends in Microbiology, Bind 28, Nr. 11, 2020, s. 913-921.Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt
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TY - JOUR
T1 - Anti-CRISPR Proteins in Archaea
AU - Peng, Xu
AU - Mayo-Muñoz, David
AU - Bhoobalan-Chitty, Yuvaraj
AU - Martínez-Álvarez, Laura
PY - 2020
Y1 - 2020
N2 - Anti-CRISPR (Acr) proteins are natural inhibitors of CRISPR-Cas immune systems. To date, Acrs inhibiting types I, II, III, V, and VI CRISPR-Cas systems have been characterized. While most known Acrs are derived from bacterial phages and prophages, very few have been characterized in the domain Archaea, despite the nearly ubiquitous presence of CRISPR-Cas in archaeal cells. Here we summarize the discovery and characterization of the archaeal Acrs with the representatives encoded by a model archaeal virus, Sulfolobus islandicus rod-shaped virus 2 (SIRV2). AcrID1 inhibits subtype I-D CRISPR-Cas immunity through direct interaction with the large subunit Cas10d of the effector complex, and AcrIIIB1 inhibits subtype III-B CRISPR-Cas immunity through a mechanism interfering with middle/late gene targeting. Future development of efficient screening methods will be key to uncovering the diversity of archaeal Acrs.
AB - Anti-CRISPR (Acr) proteins are natural inhibitors of CRISPR-Cas immune systems. To date, Acrs inhibiting types I, II, III, V, and VI CRISPR-Cas systems have been characterized. While most known Acrs are derived from bacterial phages and prophages, very few have been characterized in the domain Archaea, despite the nearly ubiquitous presence of CRISPR-Cas in archaeal cells. Here we summarize the discovery and characterization of the archaeal Acrs with the representatives encoded by a model archaeal virus, Sulfolobus islandicus rod-shaped virus 2 (SIRV2). AcrID1 inhibits subtype I-D CRISPR-Cas immunity through direct interaction with the large subunit Cas10d of the effector complex, and AcrIIIB1 inhibits subtype III-B CRISPR-Cas immunity through a mechanism interfering with middle/late gene targeting. Future development of efficient screening methods will be key to uncovering the diversity of archaeal Acrs.
KW - AcrID1
KW - AcrIIIB1
KW - anti-CRISPR (Acr)
KW - Archaea
KW - archaeal virus
KW - Sulfolobus rod-shaped virus 2 (SIRV2)
U2 - 10.1016/j.tim.2020.05.007
DO - 10.1016/j.tim.2020.05.007
M3 - Review
C2 - 32499102
AN - SCOPUS:85085644355
VL - 28
SP - 913
EP - 921
JO - Trends in Microbiology
JF - Trends in Microbiology
SN - 0966-842X
IS - 11
ER -
ID: 243152527