Analytical method for the determination of the absorption coefficient of DNA-stabilized silver nanoclusters

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Standard

Analytical method for the determination of the absorption coefficient of DNA-stabilized silver nanoclusters. / Romolini, Giacomo; Cerretani, Cecilia; Rück, Vanessa; Liisberg, Mikkel Baldtzer; Mollerup, Christian Brinch; Vosch, Tom.

I: Nanoscale, 2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Romolini, G, Cerretani, C, Rück, V, Liisberg, MB, Mollerup, CB & Vosch, T 2024, 'Analytical method for the determination of the absorption coefficient of DNA-stabilized silver nanoclusters', Nanoscale. https://doi.org/10.1039/d4nr01765j

APA

Romolini, G., Cerretani, C., Rück, V., Liisberg, M. B., Mollerup, C. B., & Vosch, T. (Accepteret/In press). Analytical method for the determination of the absorption coefficient of DNA-stabilized silver nanoclusters. Nanoscale. https://doi.org/10.1039/d4nr01765j

Vancouver

Romolini G, Cerretani C, Rück V, Liisberg MB, Mollerup CB, Vosch T. Analytical method for the determination of the absorption coefficient of DNA-stabilized silver nanoclusters. Nanoscale. 2024. https://doi.org/10.1039/d4nr01765j

Author

Romolini, Giacomo ; Cerretani, Cecilia ; Rück, Vanessa ; Liisberg, Mikkel Baldtzer ; Mollerup, Christian Brinch ; Vosch, Tom. / Analytical method for the determination of the absorption coefficient of DNA-stabilized silver nanoclusters. I: Nanoscale. 2024.

Bibtex

@article{db25f7f1aaa743b49474dd4222155f74,

title = "Analytical method for the determination of the absorption coefficient of DNA-stabilized silver nanoclusters",

abstract = "DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters formed by silver atoms and cations encapsulated in DNA oligomers. Here, we present an analytical approach to calculate the molar absorption coefficient (ϵ) of these systems, which consists of combining UV-Vis spectroscopy, electrospray ionization-mass spectrometry (ESI-MS), and inductively coupled plasma-optical emission spectrometry (ICP-OES). ESI-MS enables the determination of the number of silvers bound to the DNA strands, whereas ICP-OES allows measurement of the total amount of silver in solution. The data is used to calculate the concentration of DNA-AgNCs and together with UV-Vis absorbance, allows for the calculation of ϵ. We compare the obtained ϵ with the experimental values previously determined through fluorescence correlation spectroscopy (FCS) and theoretical estimates based on the ϵ of the DNA itself. Finally, the experimental radiative decay rates (kf) and ϵ values are evaluated and compared to those typically found for organic fluorophores, highlighting the molecular-like nature of the DNA-AgNC emission.",

author = "Giacomo Romolini and Cecilia Cerretani and Vanessa R{\"u}ck and Liisberg, {Mikkel Baldtzer} and Mollerup, {Christian Brinch} and Tom Vosch",

note = "Publisher Copyright: {\textcopyright} 2024 The Royal Society of Chemistry.",

year = "2024",

doi = "10.1039/d4nr01765j",

language = "English",

journal = "Nanoscale",

issn = "2040-3364",

publisher = "Royal Society of Chemistry",

}

RIS

TY - JOUR

T1 - Analytical method for the determination of the absorption coefficient of DNA-stabilized silver nanoclusters

AU - Romolini, Giacomo

AU - Cerretani, Cecilia

AU - Rück, Vanessa

AU - Liisberg, Mikkel Baldtzer

AU - Mollerup, Christian Brinch

AU - Vosch, Tom

PY - 2024

Y1 - 2024

N2 - DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters formed by silver atoms and cations encapsulated in DNA oligomers. Here, we present an analytical approach to calculate the molar absorption coefficient (ϵ) of these systems, which consists of combining UV-Vis spectroscopy, electrospray ionization-mass spectrometry (ESI-MS), and inductively coupled plasma-optical emission spectrometry (ICP-OES). ESI-MS enables the determination of the number of silvers bound to the DNA strands, whereas ICP-OES allows measurement of the total amount of silver in solution. The data is used to calculate the concentration of DNA-AgNCs and together with UV-Vis absorbance, allows for the calculation of ϵ. We compare the obtained ϵ with the experimental values previously determined through fluorescence correlation spectroscopy (FCS) and theoretical estimates based on the ϵ of the DNA itself. Finally, the experimental radiative decay rates (kf) and ϵ values are evaluated and compared to those typically found for organic fluorophores, highlighting the molecular-like nature of the DNA-AgNC emission.

AB - DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters formed by silver atoms and cations encapsulated in DNA oligomers. Here, we present an analytical approach to calculate the molar absorption coefficient (ϵ) of these systems, which consists of combining UV-Vis spectroscopy, electrospray ionization-mass spectrometry (ESI-MS), and inductively coupled plasma-optical emission spectrometry (ICP-OES). ESI-MS enables the determination of the number of silvers bound to the DNA strands, whereas ICP-OES allows measurement of the total amount of silver in solution. The data is used to calculate the concentration of DNA-AgNCs and together with UV-Vis absorbance, allows for the calculation of ϵ. We compare the obtained ϵ with the experimental values previously determined through fluorescence correlation spectroscopy (FCS) and theoretical estimates based on the ϵ of the DNA itself. Finally, the experimental radiative decay rates (kf) and ϵ values are evaluated and compared to those typically found for organic fluorophores, highlighting the molecular-like nature of the DNA-AgNC emission.

U2 - 10.1039/d4nr01765j

DO - 10.1039/d4nr01765j

M3 - Journal article

C2 - 38888625

AN - SCOPUS:85196538808

JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

ER -

ID: 396086132