Analysis by flow cytometry of α2-macroglobulin and nonimmune IgM-binding to Plasmodium falciparum-infected erythrocytes
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Several members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family can bind human serum proteins such as IgM and α2-macroglobulin (α2M). This binding seems to play a role in pathogenesis and immune evasion by improving the avidity of PfEMP1-mediated binding to erythrocyte receptors and/or by masking antibody epitopes in PfEMP1. In this protocol, we describe a flow cytometry-based protocol to evaluate IgM- and α2M-binding to intact and unfixed mature-stage IEs. The method can be used for laboratory clones and field isolates.
Originalsprog | Engelsk |
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Titel | Malaria Immunology : Targeting the Surface of Infected Erythrocytes |
Antal sider | 10 |
Vol/bind | 2470 |
Forlag | Humana Press |
Publikationsdato | 2022 |
Sider | 435-444 |
ISBN (Trykt) | 978-1-0716-2188-2 |
ISBN (Elektronisk) | 978-1-0716-2189-9 |
DOI | |
Status | Udgivet - 2022 |
Navn | Methods in molecular biology (Clifton, N.J.) |
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ISSN | 1064-3745 |
Bibliografisk note
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
ID: 317092512