An Assay for Studying Ubiquitylation of RNA Polymerase II and Other Proteins in Crude Yeast Extracts

Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt

Standard

An Assay for Studying Ubiquitylation of RNA Polymerase II and Other Proteins in Crude Yeast Extracts. / Reid, James; Svejstrup, Jesper Q.

I: Methods in Enzymology, Bind 408, 2006, s. 264-273.

Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt

Harvard

Reid, J & Svejstrup, JQ 2006, 'An Assay for Studying Ubiquitylation of RNA Polymerase II and Other Proteins in Crude Yeast Extracts', Methods in Enzymology, bind 408, s. 264-273. https://doi.org/10.1016/S0076-6879(06)08016-5

APA

Reid, J., & Svejstrup, J. Q. (2006). An Assay for Studying Ubiquitylation of RNA Polymerase II and Other Proteins in Crude Yeast Extracts. Methods in Enzymology, 408, 264-273. https://doi.org/10.1016/S0076-6879(06)08016-5

Vancouver

Reid J, Svejstrup JQ. An Assay for Studying Ubiquitylation of RNA Polymerase II and Other Proteins in Crude Yeast Extracts. Methods in Enzymology. 2006;408:264-273. https://doi.org/10.1016/S0076-6879(06)08016-5

Author

Reid, James ; Svejstrup, Jesper Q. / An Assay for Studying Ubiquitylation of RNA Polymerase II and Other Proteins in Crude Yeast Extracts. I: Methods in Enzymology. 2006 ; Bind 408. s. 264-273.

Bibtex

@article{e85f10851ef347569c33843ac73c0ddd,
title = "An Assay for Studying Ubiquitylation of RNA Polymerase II and Other Proteins in Crude Yeast Extracts",
abstract = "In recent years, much research effort has been invested in understanding the role and importance of protein ubiquitylation. This chapter presents protocols that enable ubiquitylation of RNAPII and other proteins to be reconstituted in crude yeast extracts in vitro. These procedures, combined with the ease of genetic manipulations in yeast, provide a new powerful tool for investigating the mechanisms underlying protein ubiquitylation.",
author = "James Reid and Svejstrup, {Jesper Q.}",
year = "2006",
doi = "10.1016/S0076-6879(06)08016-5",
language = "English",
volume = "408",
pages = "264--273",
journal = "Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods Based on Optical Imaging and Fluorescence",
issn = "0076-6879",

}

RIS

TY - JOUR

T1 - An Assay for Studying Ubiquitylation of RNA Polymerase II and Other Proteins in Crude Yeast Extracts

AU - Reid, James

AU - Svejstrup, Jesper Q.

PY - 2006

Y1 - 2006

N2 - In recent years, much research effort has been invested in understanding the role and importance of protein ubiquitylation. This chapter presents protocols that enable ubiquitylation of RNAPII and other proteins to be reconstituted in crude yeast extracts in vitro. These procedures, combined with the ease of genetic manipulations in yeast, provide a new powerful tool for investigating the mechanisms underlying protein ubiquitylation.

AB - In recent years, much research effort has been invested in understanding the role and importance of protein ubiquitylation. This chapter presents protocols that enable ubiquitylation of RNAPII and other proteins to be reconstituted in crude yeast extracts in vitro. These procedures, combined with the ease of genetic manipulations in yeast, provide a new powerful tool for investigating the mechanisms underlying protein ubiquitylation.

U2 - 10.1016/S0076-6879(06)08016-5

DO - 10.1016/S0076-6879(06)08016-5

M3 - Review

C2 - 16793374

AN - SCOPUS:33745203043

VL - 408

SP - 264

EP - 273

JO - Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods Based on Optical Imaging and Fluorescence

JF - Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods Based on Optical Imaging and Fluorescence

SN - 0076-6879

ER -

ID: 331037384