Amyloid beta-peptide(25-35) changes [Ca2+] in hippocampal neurons
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Amyloid beta-peptide(25-35) changes [Ca2+] in hippocampal neurons. / Mogensen, Helle Smidt; Beatty, D M; Morris, S J; Jorgensen, O S.
I: NeuroReport, Bind 9, Nr. 7, 1998, s. 1553-8.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Amyloid beta-peptide(25-35) changes [Ca2+] in hippocampal neurons
AU - Mogensen, Helle Smidt
AU - Beatty, D M
AU - Morris, S J
AU - Jorgensen, O S
N1 - Keywords: Amyloid beta-Protein; Animals; Animals, Newborn; Astrocytes; Benzopyrans; Calcium; Cell Survival; Cells, Cultured; Fluorescent Dyes; Glial Fibrillary Acidic Protein; Hippocampus; Hydrogen-Ion Concentration; Indoles; Kinetics; Microscopy, Fluorescence; Naphthols; Neurons; Peptide Fragments; Rats; Rhodamines; Time Factors
PY - 1998
Y1 - 1998
N2 - Insoluble aggregates of the amyloid beta-peptide (A beta) is a major constituent of senile plaques found in brains of Alzheimer disease (AD) patients. The detrimental effects of aggregated A beta is associated with an increased intracellular Ca2+ concentration ([Ca2+]i). We examined the effects of A beta(25-35) on [Ca2+]i and intracellular H+ concentration ([H+]i) in single hippocampal neurons by real time fluorescence imaging using the Ca(2+)- and H(+)-specific ratio dyes, indo-1 and SNARF-1. Incubation of these cultures with A beta(25-35) for 3-12 days in vitro increased [Ca2+]i and [H+]i in large, NMDA-responsive neurons.
AB - Insoluble aggregates of the amyloid beta-peptide (A beta) is a major constituent of senile plaques found in brains of Alzheimer disease (AD) patients. The detrimental effects of aggregated A beta is associated with an increased intracellular Ca2+ concentration ([Ca2+]i). We examined the effects of A beta(25-35) on [Ca2+]i and intracellular H+ concentration ([H+]i) in single hippocampal neurons by real time fluorescence imaging using the Ca(2+)- and H(+)-specific ratio dyes, indo-1 and SNARF-1. Incubation of these cultures with A beta(25-35) for 3-12 days in vitro increased [Ca2+]i and [H+]i in large, NMDA-responsive neurons.
M3 - Journal article
C2 - 9631466
VL - 9
SP - 1553
EP - 1558
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 7
ER -
ID: 16159446