TY - JOUR

T1 - Aluminium hydroxide adsorbed allergens used in modified RAST

AU - Poulsen, Lars K.

AU - Weeke, B

PY - 1985/8

Y1 - 1985/8

N2 - A method is described in which the conventional radioallergosorbent test (RAST) for determination of allergen-specific IgE in serum has been modified by displacing the solidphase sorbent by a gel of aluminium hydroxide (Al(OH)3). The optimum conditions for this new analysis (AlRAST) are determined, taking into account speed, costs and the ratio between responses of patients and normal individuals. The AlRAST fulfills the criteria for validity: it is specific and shows parallelism between serum dilution curves. The sensitivity and reproducibility are slightly lower than in the conventional RAST, but sufficient for most routine and screening procedures. The preparation of allergosorbents in AlRAST can be accomplished within 1 h, thus making it convenient for fast screening of patients against unusual allergen extracts, as well as for allergen standardization from dose-response curves. Further, it is possible to standardize aluminium hydroxide adsorbed extracts used for immunotherapy by using the products directly as allergosorbents in the AlRAST.

AB - A method is described in which the conventional radioallergosorbent test (RAST) for determination of allergen-specific IgE in serum has been modified by displacing the solidphase sorbent by a gel of aluminium hydroxide (Al(OH)3). The optimum conditions for this new analysis (AlRAST) are determined, taking into account speed, costs and the ratio between responses of patients and normal individuals. The AlRAST fulfills the criteria for validity: it is specific and shows parallelism between serum dilution curves. The sensitivity and reproducibility are slightly lower than in the conventional RAST, but sufficient for most routine and screening procedures. The preparation of allergosorbents in AlRAST can be accomplished within 1 h, thus making it convenient for fast screening of patients against unusual allergen extracts, as well as for allergen standardization from dose-response curves. Further, it is possible to standardize aluminium hydroxide adsorbed extracts used for immunotherapy by using the products directly as allergosorbents in the AlRAST.

KW - Allergens

KW - Aluminum Hydroxide

KW - Animals

KW - Buffers

KW - Dose-Response Relationship, Immunologic

KW - Humans

KW - Hypersensitivity

KW - Poaceae

KW - Pollen

KW - Radioallergosorbent Test

KW - Radioimmunoassay

KW - Skin

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1111/j.1398-9995.1985.tb02679.x

DO - 10.1111/j.1398-9995.1985.tb02679.x

M3 - Journal article

C2 - 4051143

VL - 40

SP - 405

EP - 416

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

SN - 0105-4538

IS - 6

ER -