Aluminium hydroxide adsorbed allergens used in modified RAST
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Aluminium hydroxide adsorbed allergens used in modified RAST. / Poulsen, Lars K.; Weeke, B.
I: Allergy, Bind 40, Nr. 6, 08.1985, s. 405-16.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Aluminium hydroxide adsorbed allergens used in modified RAST
AU - Poulsen, Lars K.
AU - Weeke, B
PY - 1985/8
Y1 - 1985/8
N2 - A method is described in which the conventional radioallergosorbent test (RAST) for determination of allergen-specific IgE in serum has been modified by displacing the solidphase sorbent by a gel of aluminium hydroxide (Al(OH)3). The optimum conditions for this new analysis (AlRAST) are determined, taking into account speed, costs and the ratio between responses of patients and normal individuals. The AlRAST fulfills the criteria for validity: it is specific and shows parallelism between serum dilution curves. The sensitivity and reproducibility are slightly lower than in the conventional RAST, but sufficient for most routine and screening procedures. The preparation of allergosorbents in AlRAST can be accomplished within 1 h, thus making it convenient for fast screening of patients against unusual allergen extracts, as well as for allergen standardization from dose-response curves. Further, it is possible to standardize aluminium hydroxide adsorbed extracts used for immunotherapy by using the products directly as allergosorbents in the AlRAST.
AB - A method is described in which the conventional radioallergosorbent test (RAST) for determination of allergen-specific IgE in serum has been modified by displacing the solidphase sorbent by a gel of aluminium hydroxide (Al(OH)3). The optimum conditions for this new analysis (AlRAST) are determined, taking into account speed, costs and the ratio between responses of patients and normal individuals. The AlRAST fulfills the criteria for validity: it is specific and shows parallelism between serum dilution curves. The sensitivity and reproducibility are slightly lower than in the conventional RAST, but sufficient for most routine and screening procedures. The preparation of allergosorbents in AlRAST can be accomplished within 1 h, thus making it convenient for fast screening of patients against unusual allergen extracts, as well as for allergen standardization from dose-response curves. Further, it is possible to standardize aluminium hydroxide adsorbed extracts used for immunotherapy by using the products directly as allergosorbents in the AlRAST.
KW - Allergens
KW - Aluminum Hydroxide
KW - Animals
KW - Buffers
KW - Dose-Response Relationship, Immunologic
KW - Humans
KW - Hypersensitivity
KW - Poaceae
KW - Pollen
KW - Radioallergosorbent Test
KW - Radioimmunoassay
KW - Skin
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1111/j.1398-9995.1985.tb02679.x
DO - 10.1111/j.1398-9995.1985.tb02679.x
M3 - Journal article
C2 - 4051143
VL - 40
SP - 405
EP - 416
JO - Allergy: European Journal of Allergy and Clinical Immunology
JF - Allergy: European Journal of Allergy and Clinical Immunology
SN - 0105-4538
IS - 6
ER -
ID: 169714822