Alternatively activated macrophages do not synthesize catecholamines or contribute to adipose tissue adaptive thermogenesis
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Alternatively activated macrophages do not synthesize catecholamines or contribute to adipose tissue adaptive thermogenesis. / Fischer, Katrin; Ruiz, Henry H; Jhun, Kevin; Finan, Brian; Oberlin, Douglas J; van der Heide, Verena; Kalinovich, Anastasia V; Petrovic, Natasa; Wolf, Yochai; Clemmensen, Christoffer; Shin, Andrew C; Divanovic, Senad; Brombacher, Frank; Glasmacher, Elke; Keipert, Susanne; Jastroch, Martin; Nagler, Joachim; Schramm, Karl-Werner; Medrikova, Dasa; Collden, Gustav; Woods, Stephen C; Herzig, Stephan; Homann, Dirk; Jung, Steffen; Nedergaard, Jan; Cannon, Barbara; Tschöp, Matthias H; Müller, Timo D; Buettner, Christoph.
I: Nature Medicine, Bind 23, Nr. 5, 05.2017, s. 623-630.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Alternatively activated macrophages do not synthesize catecholamines or contribute to adipose tissue adaptive thermogenesis
AU - Fischer, Katrin
AU - Ruiz, Henry H
AU - Jhun, Kevin
AU - Finan, Brian
AU - Oberlin, Douglas J
AU - van der Heide, Verena
AU - Kalinovich, Anastasia V
AU - Petrovic, Natasa
AU - Wolf, Yochai
AU - Clemmensen, Christoffer
AU - Shin, Andrew C
AU - Divanovic, Senad
AU - Brombacher, Frank
AU - Glasmacher, Elke
AU - Keipert, Susanne
AU - Jastroch, Martin
AU - Nagler, Joachim
AU - Schramm, Karl-Werner
AU - Medrikova, Dasa
AU - Collden, Gustav
AU - Woods, Stephen C
AU - Herzig, Stephan
AU - Homann, Dirk
AU - Jung, Steffen
AU - Nedergaard, Jan
AU - Cannon, Barbara
AU - Tschöp, Matthias H
AU - Müller, Timo D
AU - Buettner, Christoph
PY - 2017/5
Y1 - 2017/5
N2 - Adaptive thermogenesis is the process of heat generation in response to cold stimulation. It is under the control of the sympathetic nervous system, whose chief effector is the catecholamine norepinephrine (NE). NE enhances thermogenesis through β3-adrenergic receptors to activate brown adipose tissue and by 'browning' white adipose tissue. Recent studies have reported that alternative activation of macrophages in response to interleukin (IL)-4 stimulation induces the expression of tyrosine hydroxylase (TH), a key enzyme in the catecholamine synthesis pathway, and that this activation provides an alternative source of locally produced catecholamines during the thermogenic process. Here we report that the deletion of Th in hematopoietic cells of adult mice neither alters energy expenditure upon cold exposure nor reduces browning in inguinal adipose tissue. Bone marrow-derived macrophages did not release NE in response to stimulation with IL-4, and conditioned media from IL-4-stimulated macrophages failed to induce expression of thermogenic genes, such as uncoupling protein 1 (Ucp1), in adipocytes cultured with the conditioned media. Furthermore, chronic treatment with IL-4 failed to increase energy expenditure in wild-type, Ucp1-/- and interleukin-4 receptor-α double-negative (Il4ra-/-) mice. In agreement with these findings, adipose-tissue-resident macrophages did not express TH. Thus, we conclude that alternatively activated macrophages do not synthesize relevant amounts of catecholamines, and hence, are not likely to have a direct role in adipocyte metabolism or adaptive thermogenesis.
AB - Adaptive thermogenesis is the process of heat generation in response to cold stimulation. It is under the control of the sympathetic nervous system, whose chief effector is the catecholamine norepinephrine (NE). NE enhances thermogenesis through β3-adrenergic receptors to activate brown adipose tissue and by 'browning' white adipose tissue. Recent studies have reported that alternative activation of macrophages in response to interleukin (IL)-4 stimulation induces the expression of tyrosine hydroxylase (TH), a key enzyme in the catecholamine synthesis pathway, and that this activation provides an alternative source of locally produced catecholamines during the thermogenic process. Here we report that the deletion of Th in hematopoietic cells of adult mice neither alters energy expenditure upon cold exposure nor reduces browning in inguinal adipose tissue. Bone marrow-derived macrophages did not release NE in response to stimulation with IL-4, and conditioned media from IL-4-stimulated macrophages failed to induce expression of thermogenic genes, such as uncoupling protein 1 (Ucp1), in adipocytes cultured with the conditioned media. Furthermore, chronic treatment with IL-4 failed to increase energy expenditure in wild-type, Ucp1-/- and interleukin-4 receptor-α double-negative (Il4ra-/-) mice. In agreement with these findings, adipose-tissue-resident macrophages did not express TH. Thus, we conclude that alternatively activated macrophages do not synthesize relevant amounts of catecholamines, and hence, are not likely to have a direct role in adipocyte metabolism or adaptive thermogenesis.
KW - Adaptation, Physiological
KW - Adipocytes
KW - Adipose Tissue
KW - Adipose Tissue, Brown
KW - Adipose Tissue, White
KW - Animals
KW - Blotting, Western
KW - Body Composition
KW - Catecholamines
KW - Cell Differentiation
KW - Culture Media, Conditioned
KW - Energy Metabolism
KW - Flow Cytometry
KW - Fluorescent Antibody Technique
KW - Gene Expression Profiling
KW - Interleukin-4
KW - Macrophages
KW - Mice
KW - Mice, Knockout
KW - Norepinephrine
KW - Receptors, Adrenergic, beta-3
KW - Receptors, Cell Surface
KW - Thermogenesis
KW - Tyrosine 3-Monooxygenase
KW - Uncoupling Protein 1
KW - Journal Article
U2 - 10.1038/nm.4316
DO - 10.1038/nm.4316
M3 - Journal article
C2 - 28414329
VL - 23
SP - 623
EP - 630
JO - Nature Medicine
JF - Nature Medicine
SN - 1078-8956
IS - 5
ER -
ID: 186639641