Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma

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Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma. / Poulsen, Nadia Nicholine; Bjerregaard, Asger; Khoo, Siew-Kim; Laing, Ingrid A; Le Souëf, Peter; Backer, Vibeke; Rapley, Laura; Cohen, Suzanne E; Barrett, Lucy; Thompson, Philip; Baltic, Svetlana; Porsbjerg, Celeste.

I: Respiratory Medicine, Bind 140, 2018, s. 50-56.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Poulsen, NN, Bjerregaard, A, Khoo, S-K, Laing, IA, Le Souëf, P, Backer, V, Rapley, L, Cohen, SE, Barrett, L, Thompson, P, Baltic, S & Porsbjerg, C 2018, 'Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma', Respiratory Medicine, bind 140, s. 50-56. https://doi.org/10.1016/j.rmed.2018.05.016

APA

Poulsen, N. N., Bjerregaard, A., Khoo, S-K., Laing, I. A., Le Souëf, P., Backer, V., Rapley, L., Cohen, S. E., Barrett, L., Thompson, P., Baltic, S., & Porsbjerg, C. (2018). Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma. Respiratory Medicine, 140, 50-56. https://doi.org/10.1016/j.rmed.2018.05.016

Vancouver

Poulsen NN, Bjerregaard A, Khoo S-K, Laing IA, Le Souëf P, Backer V o.a. Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma. Respiratory Medicine. 2018;140:50-56. https://doi.org/10.1016/j.rmed.2018.05.016

Author

Poulsen, Nadia Nicholine ; Bjerregaard, Asger ; Khoo, Siew-Kim ; Laing, Ingrid A ; Le Souëf, Peter ; Backer, Vibeke ; Rapley, Laura ; Cohen, Suzanne E ; Barrett, Lucy ; Thompson, Philip ; Baltic, Svetlana ; Porsbjerg, Celeste. / Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma. I: Respiratory Medicine. 2018 ; Bind 140. s. 50-56.

Bibtex

@article{d61126e16f584aadb3554520e0a12e19,

title = "Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma",

abstract = "BACKGROUND: Several animal studies, and one inoculation study in adult asthmatics have shown that interleukin-33 (IL-33) is a major contributor to type-2 inflammation in acute asthma. However, the link between IL-33 and type-2 inflammation has not been shown in naturally occurring asthma exacerbations.OBJECTIVES: To determine if airway IL-33 is associated with type-2 inflammation measured by type-2 cytokines, FeNO and sputum eosinophils in patients presenting to the Emergency Department with an asthma exacerbations.METHODS: Adult patients hospitalized due to acute asthma were enrolled. Upper airways were sampled with nasal swabs and lower airways with induced sputum. Cytokines were measured at protein level using a Luminex{\textregistered} assay and mRNA expression level using droplet-digital-PCR. Airway sampling was repeated four weeks after exacerbation.RESULTS: At the time of exacerbation, upper airway IL-33 correlated with upper airway IL-5 and IL-13 (R = 0.84, p < 0.01 and R = 0.76, p < 0.01, respectively) and with lower airway IL-13 (R = 0.49, p = 0.03). Similar associations were observed for mRNA expression. Lower airway IL-33 positively correlated with lower airway IL-13 (R = 0.84, p < 0.01). IL-13 and IL-33 were positively correlated with FeNO, and IL-5 with eosinophils. The association between IL-33 and type-2 cytokines were still present four weeks after exacerbation.CONCLUSION: This is the first study to demonstrate that airway IL-33 is associated with type-2 cytokines in naturally occurring asthma exacerbations in adults, providing in vivo evidence supporting that IL-33 may be driving type-2 inflammation in acute asthma. Thus supporting IL-33 as a potential future drug target due to its role, upstream in the immunological cascade.",

keywords = "Acute Disease, Adult, Asthma/immunology, Cytokines/genetics, Eosinophils/immunology, Female, Follow-Up Studies, Gene Expression/immunology, Humans, Inflammation Mediators/metabolism, Interleukin-13/genetics, Interleukin-33/genetics, Interleukin-5/genetics, Male, Middle Aged, Nasal Mucosa/immunology, RNA, Messenger/genetics, Severity of Illness Index, Sputum/immunology, Young Adult",

author = "Poulsen, {Nadia Nicholine} and Asger Bjerregaard and Siew-Kim Khoo and Laing, {Ingrid A} and {Le Sou{\"e}f}, Peter and Vibeke Backer and Laura Rapley and Cohen, {Suzanne E} and Lucy Barrett and Philip Thompson and Svetlana Baltic and Celeste Porsbjerg",

year = "2018",

doi = "10.1016/j.rmed.2018.05.016",

language = "English",

volume = "140",

pages = "50--56",

journal = "Respiratory Medicine",

issn = "0954-6111",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Airway Interleukin-33 and type 2 cytokines in adult patients with acute asthma

AU - Poulsen, Nadia Nicholine

AU - Bjerregaard, Asger

AU - Khoo, Siew-Kim

AU - Laing, Ingrid A

AU - Le Souëf, Peter

AU - Backer, Vibeke

AU - Rapley, Laura

AU - Cohen, Suzanne E

AU - Barrett, Lucy

AU - Thompson, Philip

AU - Baltic, Svetlana

AU - Porsbjerg, Celeste

PY - 2018

Y1 - 2018

N2 - BACKGROUND: Several animal studies, and one inoculation study in adult asthmatics have shown that interleukin-33 (IL-33) is a major contributor to type-2 inflammation in acute asthma. However, the link between IL-33 and type-2 inflammation has not been shown in naturally occurring asthma exacerbations.OBJECTIVES: To determine if airway IL-33 is associated with type-2 inflammation measured by type-2 cytokines, FeNO and sputum eosinophils in patients presenting to the Emergency Department with an asthma exacerbations.METHODS: Adult patients hospitalized due to acute asthma were enrolled. Upper airways were sampled with nasal swabs and lower airways with induced sputum. Cytokines were measured at protein level using a Luminex® assay and mRNA expression level using droplet-digital-PCR. Airway sampling was repeated four weeks after exacerbation.RESULTS: At the time of exacerbation, upper airway IL-33 correlated with upper airway IL-5 and IL-13 (R = 0.84, p < 0.01 and R = 0.76, p < 0.01, respectively) and with lower airway IL-13 (R = 0.49, p = 0.03). Similar associations were observed for mRNA expression. Lower airway IL-33 positively correlated with lower airway IL-13 (R = 0.84, p < 0.01). IL-13 and IL-33 were positively correlated with FeNO, and IL-5 with eosinophils. The association between IL-33 and type-2 cytokines were still present four weeks after exacerbation.CONCLUSION: This is the first study to demonstrate that airway IL-33 is associated with type-2 cytokines in naturally occurring asthma exacerbations in adults, providing in vivo evidence supporting that IL-33 may be driving type-2 inflammation in acute asthma. Thus supporting IL-33 as a potential future drug target due to its role, upstream in the immunological cascade.

AB - BACKGROUND: Several animal studies, and one inoculation study in adult asthmatics have shown that interleukin-33 (IL-33) is a major contributor to type-2 inflammation in acute asthma. However, the link between IL-33 and type-2 inflammation has not been shown in naturally occurring asthma exacerbations.OBJECTIVES: To determine if airway IL-33 is associated with type-2 inflammation measured by type-2 cytokines, FeNO and sputum eosinophils in patients presenting to the Emergency Department with an asthma exacerbations.METHODS: Adult patients hospitalized due to acute asthma were enrolled. Upper airways were sampled with nasal swabs and lower airways with induced sputum. Cytokines were measured at protein level using a Luminex® assay and mRNA expression level using droplet-digital-PCR. Airway sampling was repeated four weeks after exacerbation.RESULTS: At the time of exacerbation, upper airway IL-33 correlated with upper airway IL-5 and IL-13 (R = 0.84, p < 0.01 and R = 0.76, p < 0.01, respectively) and with lower airway IL-13 (R = 0.49, p = 0.03). Similar associations were observed for mRNA expression. Lower airway IL-33 positively correlated with lower airway IL-13 (R = 0.84, p < 0.01). IL-13 and IL-33 were positively correlated with FeNO, and IL-5 with eosinophils. The association between IL-33 and type-2 cytokines were still present four weeks after exacerbation.CONCLUSION: This is the first study to demonstrate that airway IL-33 is associated with type-2 cytokines in naturally occurring asthma exacerbations in adults, providing in vivo evidence supporting that IL-33 may be driving type-2 inflammation in acute asthma. Thus supporting IL-33 as a potential future drug target due to its role, upstream in the immunological cascade.

KW - Acute Disease

KW - Adult

KW - Asthma/immunology

KW - Cytokines/genetics

KW - Eosinophils/immunology

KW - Female

KW - Follow-Up Studies

KW - Gene Expression/immunology

KW - Humans

KW - Inflammation Mediators/metabolism

KW - Interleukin-13/genetics

KW - Interleukin-33/genetics

KW - Interleukin-5/genetics

KW - Male

KW - Middle Aged

KW - Nasal Mucosa/immunology

KW - RNA, Messenger/genetics

KW - Severity of Illness Index

KW - Sputum/immunology

KW - Young Adult

U2 - 10.1016/j.rmed.2018.05.016

DO - 10.1016/j.rmed.2018.05.016

M3 - Journal article

C2 - 29957280

VL - 140

SP - 50

EP - 56

JO - Respiratory Medicine

JF - Respiratory Medicine

SN - 0954-6111

ER -

ID: 213153974