Agarose-based gel electromembrane extraction using silica nanoparticles coated with polymeric deep eutectic solvent as a membrane additive
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Agarose-based gel electromembrane extraction using silica nanoparticles coated with polymeric deep eutectic solvent as a membrane additive. / Zeraatkar Moghaddam, Ali; Tabani, Hadi; Bameri, Amirehsan; Arabi, Elahe; Alexovič, Michal; Pedersen-Bjergaard, Stig.
I: Journal of Molecular Liquids, Bind 401, 124615, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Agarose-based gel electromembrane extraction using silica nanoparticles coated with polymeric deep eutectic solvent as a membrane additive
AU - Zeraatkar Moghaddam, Ali
AU - Tabani, Hadi
AU - Bameri, Amirehsan
AU - Arabi, Elahe
AU - Alexovič, Michal
AU - Pedersen-Bjergaard, Stig
N1 - Publisher Copyright: © 2024
PY - 2024
Y1 - 2024
N2 - The suggested work brings a novel knowledge of an electric-induced mass transfer occurring during the gel electro-membrane extraction (G-EME) followed by HPLC UV detection. The natural deep eutectic solvent(s) (DESs), such as choline chloride mixed with itaconic acid (mole ratio 1:1) and choline chloride mixed with methacrylic acid (mole ratio 1:1), were used as the green additives in the agarose gel membrane. The impact of DESs was tested for the extraction of codeine, dasatinib, imatinib, morphine, and nilotinib from human plasma and urine samples. The DESs were incorporated into silica nanoparticles (SiNPs) through porous polymerization to form a SiNPs@P(DES), subsequently dispersed in the gel structure. As a result, the agarose membrane was stabilized, extraction efficiency increased, and the EEO flow diminished. In addition, testing of nonporous polymerization versus porous polymerization showed that extraction efficiencies are higher using the latter approach due to the higher surface area of the porous SiNPs@P(DES). The optimal extraction conditions were found to be 3.0 % w/v agarose gel (pH 3.5) containing 0.02 % w/v SiNPs@P(DES), applied voltage at 60 V, extraction time at 10 min, pH of the donor phase at 6.0, and pH of the acceptor phase at 4.0. The obtained extraction recoveries were in the range of 88.1–92.9 %. The limits of detection (LODs) and quantification (LOQs) were 1.6–14.5 ng mL−1 and 5.3–47.8 ng mL−1, respectively. The intra- and inter-day repeatability (n = 4) were within 3.3 % and 6.4 % RSD, respectively.
AB - The suggested work brings a novel knowledge of an electric-induced mass transfer occurring during the gel electro-membrane extraction (G-EME) followed by HPLC UV detection. The natural deep eutectic solvent(s) (DESs), such as choline chloride mixed with itaconic acid (mole ratio 1:1) and choline chloride mixed with methacrylic acid (mole ratio 1:1), were used as the green additives in the agarose gel membrane. The impact of DESs was tested for the extraction of codeine, dasatinib, imatinib, morphine, and nilotinib from human plasma and urine samples. The DESs were incorporated into silica nanoparticles (SiNPs) through porous polymerization to form a SiNPs@P(DES), subsequently dispersed in the gel structure. As a result, the agarose membrane was stabilized, extraction efficiency increased, and the EEO flow diminished. In addition, testing of nonporous polymerization versus porous polymerization showed that extraction efficiencies are higher using the latter approach due to the higher surface area of the porous SiNPs@P(DES). The optimal extraction conditions were found to be 3.0 % w/v agarose gel (pH 3.5) containing 0.02 % w/v SiNPs@P(DES), applied voltage at 60 V, extraction time at 10 min, pH of the donor phase at 6.0, and pH of the acceptor phase at 4.0. The obtained extraction recoveries were in the range of 88.1–92.9 %. The limits of detection (LODs) and quantification (LOQs) were 1.6–14.5 ng mL−1 and 5.3–47.8 ng mL−1, respectively. The intra- and inter-day repeatability (n = 4) were within 3.3 % and 6.4 % RSD, respectively.
KW - Biological samples
KW - Drug analysis
KW - Green additive
KW - Modified gel
KW - Sample preparation
KW - Supporting materials
U2 - 10.1016/j.molliq.2024.124615
DO - 10.1016/j.molliq.2024.124615
M3 - Journal article
AN - SCOPUS:85190068720
VL - 401
JO - Journal of Molecular Liquids
JF - Journal of Molecular Liquids
SN - 0167-7322
M1 - 124615
ER -
ID: 388943316