Aescin - a natural soap for the formation of lipid nanodiscs with tunable size
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Aescin - a natural soap for the formation of lipid nanodiscs with tunable size. / Geisler, Ramsia; Pedersen, Martin Cramer; Preisig, Natalie; Hannappel, Yvonne; Prevost, Sylvain; Dattani, Rajeev; Arleth, Lise; Hellweg, Thomas.
I: Soft Matter, Bind 17, Nr. 7, 21.02.2021, s. 1888-1900.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Aescin - a natural soap for the formation of lipid nanodiscs with tunable size
AU - Geisler, Ramsia
AU - Pedersen, Martin Cramer
AU - Preisig, Natalie
AU - Hannappel, Yvonne
AU - Prevost, Sylvain
AU - Dattani, Rajeev
AU - Arleth, Lise
AU - Hellweg, Thomas
PY - 2021/2/21
Y1 - 2021/2/21
N2 - The saponin beta-aescin from the seed extract of the horse chestnut tree Aesculus hippocastanum has demonstrated a beneficial role in clinical therapy which is in part related to its strong interaction with biological membranes. In this context the present work investigates the self-assembly of nm-sized discoidal lipid nanoparticles composed of beta-aescin and the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). The discoidal lipid nanoparticles reassemble from small discs into larger discs, ribbons and finally stacks of sheets upon heating from gel-phase to fluid phase DMPC. The morphological transition of the lipid nano-particles is mainly triggered by the phospholipid phase state change. The final morphology depends on the phospholipid-to-saponin ratio and the actual temperature. The study is conducted by small-angle X-ray scattering (SAXS) and transmission (TEM) and freeze fracture electron microscopy (FFEM) are used to cover larger length scales. Two different models, representing a disc and ribbon-like shape are applied to the SAXS data, evaluating possible geometries and molecular mixing of the nano-particles. The stacked sheets are analysed by the Caille theory.
AB - The saponin beta-aescin from the seed extract of the horse chestnut tree Aesculus hippocastanum has demonstrated a beneficial role in clinical therapy which is in part related to its strong interaction with biological membranes. In this context the present work investigates the self-assembly of nm-sized discoidal lipid nanoparticles composed of beta-aescin and the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). The discoidal lipid nanoparticles reassemble from small discs into larger discs, ribbons and finally stacks of sheets upon heating from gel-phase to fluid phase DMPC. The morphological transition of the lipid nano-particles is mainly triggered by the phospholipid phase state change. The final morphology depends on the phospholipid-to-saponin ratio and the actual temperature. The study is conducted by small-angle X-ray scattering (SAXS) and transmission (TEM) and freeze fracture electron microscopy (FFEM) are used to cover larger length scales. Two different models, representing a disc and ribbon-like shape are applied to the SAXS data, evaluating possible geometries and molecular mixing of the nano-particles. The stacked sheets are analysed by the Caille theory.
U2 - 10.1039/d0sm02043e
DO - 10.1039/d0sm02043e
M3 - Journal article
C2 - 33410858
VL - 17
SP - 1888
EP - 1900
JO - Journal of Materials Chemistry
JF - Journal of Materials Chemistry
SN - 1744-683X
IS - 7
ER -
ID: 258657794