[ADMAdda5]-microcystins in Planktothrix agardhii strain PH-123 (cyanobacteria) - importance for monitoring of microcystins in the environment
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[ADMAdda5]-microcystins in Planktothrix agardhii strain PH-123 (cyanobacteria) - importance for monitoring of microcystins in the environment. / Laub, J.; Henriksen, P.; Brittain, S.M.; Wang, J.; Carmichael, W.W.; Reinehart, K.L.; Moestrup, Øjvind.
I: Environmental Toxicology (Print Edition), Bind 17, Nr. 4, 2002, s. 351-357.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - [ADMAdda5]-microcystins in Planktothrix agardhii strain PH-123 (cyanobacteria) - importance for monitoring of microcystins in the environment
AU - Laub, J.
AU - Henriksen, P.
AU - Brittain, S.M.
AU - Wang, J.
AU - Carmichael, W.W.
AU - Reinehart, K.L.
AU - Moestrup, Øjvind
N1 - Keywords cyanobacteria • Planktothrix • hepatotoxins • microcystins • protein phosphatase inhibition
PY - 2002
Y1 - 2002
N2 - Two major and two minor microcystins (MCYST) were isolated from a hepatotoxic Danish strain of Planktothrix agardhii (Gomont) Anagnostidis et Komárek by reversed-phase high-performance liquid chromatography. The microcystins were characterized by UV spectroscopy, amino acid analysis, fast atom bombardment mass spectrometry (FABMS), and high-resolution FABMS. The major microcystins were further analysed by collisionally induced tandem electrospray ionization MS. The microcystins were found to be demethylated variants of MCYST-HtyR (homotyrosine-arginine) and MCYST-LR (leucine-arginine). The two major microcystins contained an acetyl-demethyl variant (ADMAdda) of 3-amino-9-acetoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid (Adda). This is the first report of [ADMAdda5]-microcystins in Planktothrix. The two [ADMAdda5]-microcystins inhibited protein phosphatase activity but showed low cross-reactivity with antibodies of an enzyme-linked immunosorbent assay (ELISA), emphasizing the potential underestimation of the toxicity of natural blooms dominated by Planktothrix when microcystin content is quantified using only an ELISA. © 2002 Wiley Periodicals, Inc. Environ Toxicol 17: 351-357, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/tox.10042
AB - Two major and two minor microcystins (MCYST) were isolated from a hepatotoxic Danish strain of Planktothrix agardhii (Gomont) Anagnostidis et Komárek by reversed-phase high-performance liquid chromatography. The microcystins were characterized by UV spectroscopy, amino acid analysis, fast atom bombardment mass spectrometry (FABMS), and high-resolution FABMS. The major microcystins were further analysed by collisionally induced tandem electrospray ionization MS. The microcystins were found to be demethylated variants of MCYST-HtyR (homotyrosine-arginine) and MCYST-LR (leucine-arginine). The two major microcystins contained an acetyl-demethyl variant (ADMAdda) of 3-amino-9-acetoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid (Adda). This is the first report of [ADMAdda5]-microcystins in Planktothrix. The two [ADMAdda5]-microcystins inhibited protein phosphatase activity but showed low cross-reactivity with antibodies of an enzyme-linked immunosorbent assay (ELISA), emphasizing the potential underestimation of the toxicity of natural blooms dominated by Planktothrix when microcystin content is quantified using only an ELISA. © 2002 Wiley Periodicals, Inc. Environ Toxicol 17: 351-357, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/tox.10042
U2 - 10.1002/tox.10042
DO - 10.1002/tox.10042
M3 - Journal article
VL - 17
SP - 351
EP - 357
JO - Environmental Toxicology
JF - Environmental Toxicology
SN - 1520-4081
IS - 4
ER -
ID: 131524