Additive manufacturing of polymeric scaffolds for biomimetic cell membrane engineering
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Additive manufacturing of polymeric scaffolds for biomimetic cell membrane engineering. / Rovira, David Sabate; Nielsen, Hanne Morck; Taboryski, Rafael; Bunea, Ada-Ioana.
I: Materials and Design, Bind 201, 109486, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Additive manufacturing of polymeric scaffolds for biomimetic cell membrane engineering
AU - Rovira, David Sabate
AU - Nielsen, Hanne Morck
AU - Taboryski, Rafael
AU - Bunea, Ada-Ioana
PY - 2021
Y1 - 2021
N2 - Additive manufacturing based on direct laser writing two-photon polymerization facilitates the fabrication of microstructures with full 3D design freedom. Here, this fabrication technique is exploited for engineering scaffolds accurately mimicking the shape and size of three types of human cells. The human cell models employed in the study were chosen to include a range of dimensions and different identifiable features to highlight the versatility of this fabrication approach, yet other cell shapes can easily be fabricated in similar manner. The design and fabrication parameters for the additive manufacturing process were optimized to obtain polymeric scaffolds with biomimetic shapes. After fabrication, the cell scaffolds were converted to polymer-cushioned model cell membranes through layer-by layer functionalization with a cationic polymer and a lipid bilayer. Scaffold functionalization was verified using con focal laser scanning microscopy. Polymer-cushioned model cell membranes supported on 3D scaffolds mimicking the shape of human cells are particularly suitable for membrane interaction studies where membrane curvature plays an important role. The aim of this study is to demonstrate the engineering of biomimetic cell membranes by high resolution additive manufacturing combined with surface functionalization. The interdisciplinary approach highlights the value of additive manufacturing as technological solution for challenges encountered in biomedical studies.(c) 2021 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).
AB - Additive manufacturing based on direct laser writing two-photon polymerization facilitates the fabrication of microstructures with full 3D design freedom. Here, this fabrication technique is exploited for engineering scaffolds accurately mimicking the shape and size of three types of human cells. The human cell models employed in the study were chosen to include a range of dimensions and different identifiable features to highlight the versatility of this fabrication approach, yet other cell shapes can easily be fabricated in similar manner. The design and fabrication parameters for the additive manufacturing process were optimized to obtain polymeric scaffolds with biomimetic shapes. After fabrication, the cell scaffolds were converted to polymer-cushioned model cell membranes through layer-by layer functionalization with a cationic polymer and a lipid bilayer. Scaffold functionalization was verified using con focal laser scanning microscopy. Polymer-cushioned model cell membranes supported on 3D scaffolds mimicking the shape of human cells are particularly suitable for membrane interaction studies where membrane curvature plays an important role. The aim of this study is to demonstrate the engineering of biomimetic cell membranes by high resolution additive manufacturing combined with surface functionalization. The interdisciplinary approach highlights the value of additive manufacturing as technological solution for challenges encountered in biomedical studies.(c) 2021 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).
KW - Additive manufacturing
KW - Two-photon polymerization
KW - 3D design
KW - Model cell membrane
KW - Surface modification
U2 - 10.1016/j.matdes.2021.109486
DO - 10.1016/j.matdes.2021.109486
M3 - Journal article
VL - 201
JO - Materials and Design
JF - Materials and Design
SN - 0264-1275
M1 - 109486
ER -
ID: 261217555