Activity-dependent volume transmission by transgene NPY attenuates glutamate release and LTP in the subiculum
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Activity-dependent volume transmission by transgene NPY attenuates glutamate release and LTP in the subiculum. / Sørensen, Andreas T; Kanter-Schlifke, Irene; Lin, En-Ju D; During, Matthew J; Kokaia, Merab.
I: Molecular and Cellular Neuroscience, Bind 39, Nr. 2, 10.2008, s. 229-37.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Activity-dependent volume transmission by transgene NPY attenuates glutamate release and LTP in the subiculum
AU - Sørensen, Andreas T
AU - Kanter-Schlifke, Irene
AU - Lin, En-Ju D
AU - During, Matthew J
AU - Kokaia, Merab
PY - 2008/10
Y1 - 2008/10
N2 - Neuropeptide Y (NPY) gene transduction of the brain using viral vectors in epileptogenic regions can effectively suppress seizures in animals, and is being considered as a promising alternative treatment strategy for epilepsy. Therefore, it is fundamental to understand the detailed mechanisms governing the release and action of transgene NPY in neuronal circuitries. Using whole-cell recordings from subicular neurons, we show that in animals transduced by recombinant adeno-associated viral (rAAV) vector carrying the NPY gene, transgene NPY is released during high-frequency activation of CA1-subicular synapses. Released transgene NPY attenuates excitatory synaptic transmission not only in activated, but also in neighboring, non-activated synapses. Such broad action of transgene NPY may prevent recruitment of excitatory synapses in epileptic activity and could play a key role in limiting the spread and generalization of seizures.
AB - Neuropeptide Y (NPY) gene transduction of the brain using viral vectors in epileptogenic regions can effectively suppress seizures in animals, and is being considered as a promising alternative treatment strategy for epilepsy. Therefore, it is fundamental to understand the detailed mechanisms governing the release and action of transgene NPY in neuronal circuitries. Using whole-cell recordings from subicular neurons, we show that in animals transduced by recombinant adeno-associated viral (rAAV) vector carrying the NPY gene, transgene NPY is released during high-frequency activation of CA1-subicular synapses. Released transgene NPY attenuates excitatory synaptic transmission not only in activated, but also in neighboring, non-activated synapses. Such broad action of transgene NPY may prevent recruitment of excitatory synapses in epileptic activity and could play a key role in limiting the spread and generalization of seizures.
KW - Animals
KW - Animals, Newborn
KW - Arginine
KW - Benzazepines
KW - Biophysical Phenomena
KW - Chi-Square Distribution
KW - Electric Stimulation
KW - Excitatory Postsynaptic Potentials
KW - Female
KW - GABA Antagonists
KW - Genetic Vectors
KW - Glutamic Acid
KW - Green Fluorescent Proteins
KW - Hippocampus
KW - In Vitro Techniques
KW - Long-Term Potentiation
KW - Male
KW - Neurons
KW - Neuropeptide Y
KW - Patch-Clamp Techniques
KW - Phosphopyruvate Hydratase
KW - Picrotoxin
KW - Rats
KW - Rats, Sprague-Dawley
KW - Receptors, Neuropeptide Y
KW - Tetrodotoxin
KW - Transgenes
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.mcn.2008.06.014
DO - 10.1016/j.mcn.2008.06.014
M3 - Journal article
C2 - 18652899
VL - 39
SP - 229
EP - 237
JO - Molecular and Cellular Neurosciences
JF - Molecular and Cellular Neurosciences
SN - 1044-7431
IS - 2
ER -
ID: 186411872