TY - JOUR

T1 - ABO, secretor, and Lewis carbohydrate histo-blood groups are associated with autoimmune neutropenia of early childhood in Danish patients

AU - Kløve-Mogensen, Kirstine

AU - Steffensen, Rudi

AU - Masmas, Tania Nicole

AU - Glenthøj, Andreas

AU - Haunstrup, Thure Mors

AU - Ratcliffe, Paul

AU - Höglund, Petter

AU - Hasle, Henrik

AU - Nielsen, Kaspar René

N1 - Publisher Copyright: © 2022 The Authors. Transfusion published by Wiley Periodicals LLC on behalf of AABB.

PY - 2022

Y1 - 2022

N2 - Background: Autoimmune neutropenia of early childhood (AIN) is caused by autoantibodies directed against antigens on the neutrophil membrane. The ABO, secretor, and Lewis histo-blood group systems control the expression of carbohydrate antigens and have previously been linked to autoimmune diseases. We aimed to investigate the association between genotypes and the risk of AIN in Danish patients. Study Design and Methods: One hundred fifty-four antibody-positive AIN patients were included. Controls (n = 400) were healthy unrelated Danish blood donors. Molecular determination of ABO, secretor (FUT2), and Lewis (FUT3) genotypes were determined using real-time polymerase chain reaction (qPCR) or Sanger sequencing to infer the prevalence of Lewis antigens (Lea and Leb) and secretor (SeSe or Sese) or nonsecretor (sese) phenotypes. Results: Blood type O was more common in controls (46.8%) than in AIN patients (36.4%) (OR = 0.65; p = 0.028). Secretors of H Leb antigens were less frequent among AIN patients (25.2%) than controls (35.0%) (OR = 0.62; p = 0.037). Discussion: ABO blood group antigens and the secretion of these antigens are associated with a diagnosis of AIN. The mechanism underlying the association between autoimmunity and interaction among ABO, secretor, and Lewis genotypes has not yet been elucidated, but several studies indicate a connection to the gut microbiota.

AB - Background: Autoimmune neutropenia of early childhood (AIN) is caused by autoantibodies directed against antigens on the neutrophil membrane. The ABO, secretor, and Lewis histo-blood group systems control the expression of carbohydrate antigens and have previously been linked to autoimmune diseases. We aimed to investigate the association between genotypes and the risk of AIN in Danish patients. Study Design and Methods: One hundred fifty-four antibody-positive AIN patients were included. Controls (n = 400) were healthy unrelated Danish blood donors. Molecular determination of ABO, secretor (FUT2), and Lewis (FUT3) genotypes were determined using real-time polymerase chain reaction (qPCR) or Sanger sequencing to infer the prevalence of Lewis antigens (Lea and Leb) and secretor (SeSe or Sese) or nonsecretor (sese) phenotypes. Results: Blood type O was more common in controls (46.8%) than in AIN patients (36.4%) (OR = 0.65; p = 0.028). Secretors of H Leb antigens were less frequent among AIN patients (25.2%) than controls (35.0%) (OR = 0.62; p = 0.037). Discussion: ABO blood group antigens and the secretion of these antigens are associated with a diagnosis of AIN. The mechanism underlying the association between autoimmunity and interaction among ABO, secretor, and Lewis genotypes has not yet been elucidated, but several studies indicate a connection to the gut microbiota.

KW - AIN

KW - histo-blood group

KW - Lewis

KW - polymorphisms of FUT2 and FUT3

KW - secretor status

U2 - 10.1111/trf.17002

DO - 10.1111/trf.17002

M3 - Journal article

C2 - 35792132

AN - SCOPUS:85133489512

VL - 62

SP - 1636

EP - 1642

JO - Transfusion

JF - Transfusion

SN - 0041-1132

IS - 8

ER -