A synchronized, large-scale field experiment using Arabidopsis thaliana reveals the significance of the UV-B photoreceptor UVR8 under natural conditions
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A synchronized, large-scale field experiment using Arabidopsis thaliana reveals the significance of the UV-B photoreceptor UVR8 under natural conditions. / Neugart, Susanne; Steininger, Viktoria; Fernandes, Catarina; Martínez-Abaigar, Javier; Núñez-Olivera, Encarnación; Schreiner, Monika; Strid, Åke; Viczián, András; Albert, Andreas; Badenes-Pérez, Francisco R.; Castagna, Antonella; Dáder, Beatriz; Fereres, Alberto; Gaberscik, Alenka; Gulyás, Ágnes; Gwynn-Jones, Dylan; Nagy, Ferenc; Jones, Alan; Julkunen-Tiitto, Riitta; Konstantinova, Nataliia; Lakkala, Kaisa; Llorens, Laura; Martínez-Lüscher, Johann; Nybakken, Line; Olsen, Jorunn; Pascual, Inmaculada; Ranieri, Annamaria; Regier, Nicole; Robson, Matthew; Rosenqvist, Eva; Santin, Marco; Turunen, Minna; Vandenbussche, Filip; Verdaguer, Dolors; Winkler, Barbro; Witzel, Katja; Grifoni, Daniele; Zipoli, Gaetano; Hideg, Éva; Jansen, Marcel A.K.; Hauser, Marie Theres.
I: Plant Cell and Environment, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - A synchronized, large-scale field experiment using Arabidopsis thaliana reveals the significance of the UV-B photoreceptor UVR8 under natural conditions
AU - Neugart, Susanne
AU - Steininger, Viktoria
AU - Fernandes, Catarina
AU - Martínez-Abaigar, Javier
AU - Núñez-Olivera, Encarnación
AU - Schreiner, Monika
AU - Strid, Åke
AU - Viczián, András
AU - Albert, Andreas
AU - Badenes-Pérez, Francisco R.
AU - Castagna, Antonella
AU - Dáder, Beatriz
AU - Fereres, Alberto
AU - Gaberscik, Alenka
AU - Gulyás, Ágnes
AU - Gwynn-Jones, Dylan
AU - Nagy, Ferenc
AU - Jones, Alan
AU - Julkunen-Tiitto, Riitta
AU - Konstantinova, Nataliia
AU - Lakkala, Kaisa
AU - Llorens, Laura
AU - Martínez-Lüscher, Johann
AU - Nybakken, Line
AU - Olsen, Jorunn
AU - Pascual, Inmaculada
AU - Ranieri, Annamaria
AU - Regier, Nicole
AU - Robson, Matthew
AU - Rosenqvist, Eva
AU - Santin, Marco
AU - Turunen, Minna
AU - Vandenbussche, Filip
AU - Verdaguer, Dolors
AU - Winkler, Barbro
AU - Witzel, Katja
AU - Grifoni, Daniele
AU - Zipoli, Gaetano
AU - Hideg, Éva
AU - Jansen, Marcel A.K.
AU - Hauser, Marie Theres
N1 - Publisher Copyright: © 2024 The Author(s). Plant, Cell & Environment published by John Wiley & Sons Ltd.
PY - 2024
Y1 - 2024
N2 - This study determines the functional role of the plant ultraviolet-B radiation (UV-B) photoreceptor, UV RESISTANCE LOCUS 8 (UVR8) under natural conditions using a large-scale ‘synchronized-genetic-perturbation-field-experiment’. Laboratory experiments have demonstrated a role for UVR8 in UV-B responses but do not reflect the complexity of outdoor conditions where ‘genotype × environment’ interactions can mask laboratory-observed responses. Arabidopsis thaliana knockout mutant, uvr8-7, and the corresponding Wassilewskija wild type, were sown outdoors on the same date at 21 locations across Europe, ranging from 39°N to 67°N latitude. Growth and climatic data were monitored until bolting. At the onset of bolting, rosette size, dry weight, and phenolics and glucosinolates were quantified. The uvr8-7 mutant developed a larger rosette and contained less kaempferol glycosides, quercetin glycosides and hydroxycinnamic acid derivatives than the wild type across all locations, demonstrating a role for UVR8 under field conditions. UV effects on rosette size and kaempferol glycoside content were UVR8 dependent, but independent of latitude. In contrast, differences between wild type and uvr8-7 in total quercetin glycosides, and the quercetin-to-kaempferol ratio decreased with increasing latitude, that is, a more variable UV response. Thus, the large-scale synchronized approach applied demonstrates a location-dependent functional role of UVR8 under natural conditions.
AB - This study determines the functional role of the plant ultraviolet-B radiation (UV-B) photoreceptor, UV RESISTANCE LOCUS 8 (UVR8) under natural conditions using a large-scale ‘synchronized-genetic-perturbation-field-experiment’. Laboratory experiments have demonstrated a role for UVR8 in UV-B responses but do not reflect the complexity of outdoor conditions where ‘genotype × environment’ interactions can mask laboratory-observed responses. Arabidopsis thaliana knockout mutant, uvr8-7, and the corresponding Wassilewskija wild type, were sown outdoors on the same date at 21 locations across Europe, ranging from 39°N to 67°N latitude. Growth and climatic data were monitored until bolting. At the onset of bolting, rosette size, dry weight, and phenolics and glucosinolates were quantified. The uvr8-7 mutant developed a larger rosette and contained less kaempferol glycosides, quercetin glycosides and hydroxycinnamic acid derivatives than the wild type across all locations, demonstrating a role for UVR8 under field conditions. UV effects on rosette size and kaempferol glycoside content were UVR8 dependent, but independent of latitude. In contrast, differences between wild type and uvr8-7 in total quercetin glycosides, and the quercetin-to-kaempferol ratio decreased with increasing latitude, that is, a more variable UV response. Thus, the large-scale synchronized approach applied demonstrates a location-dependent functional role of UVR8 under natural conditions.
KW - flavonoid
KW - glucosinolate
KW - metabolite
KW - plant morphology
U2 - 10.1111/pce.15008
DO - 10.1111/pce.15008
M3 - Journal article
C2 - 38881245
AN - SCOPUS:85196141757
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
SN - 0140-7791
ER -
ID: 396406350