A Representative Collection of Commensal Extended-Spectrum- and AmpC-β-Lactamase-Producing Escherichia coli of Animal Origin for Phage Sensitivity Studies
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A Representative Collection of Commensal Extended-Spectrum- and AmpC-β-Lactamase-Producing Escherichia coli of Animal Origin for Phage Sensitivity Studies. / Vitt, Amira R.; Sørensen, Martine C.Holst; Bortolaia, Valeria; Brøndsted, Lone.
I: PHAGE: Therapy, Applications, and Research, Bind 4, Nr. 1, 2023, s. 35-45.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - A Representative Collection of Commensal Extended-Spectrum- and AmpC-β-Lactamase-Producing Escherichia coli of Animal Origin for Phage Sensitivity Studies
AU - Vitt, Amira R.
AU - Sørensen, Martine C.Holst
AU - Bortolaia, Valeria
AU - Brøndsted, Lone
N1 - Publisher Copyright: © 2023, Mary Ann Liebert, Inc., publishers.
PY - 2023
Y1 - 2023
N2 - Introduction: Extended-spectrum β-lactamase (ESBL)- and AmpC β-lactamase (AmpC)-producing Escherichia coli from livestock and meat represent a zoonotic risk and biocontrol solutions are needed to prevent transmission to humans. Methods: In this study, we established a representative collection of animal-origin ESBL/AmpC E. coli as target to test the antimicrobial potential of bacteriophages. Results: Bioinformatic analysis of whole-genome sequence data of 198 ESBL/AmpC E. coli from pigs, broilers, and broiler meat identified strains belonging to all known E. coli phylogroups and 65 multilocus sequence types. Various ESBL/AmpC genes and plasmid types were detected with expected source-specific patterns. Plaque assay using 15 phages previously isolated using the E. coli reference collection demonstrated that Warwickvirus phages showed the broadest host range, killing up to 26 strains. Conclusions:154/198 strains were resistant to infection by all phages tested, suggesting a need for isolating phages specific for ESBL/AmpC E. coli. The strain collection described in this study is a useful resource fulfilling such need.
AB - Introduction: Extended-spectrum β-lactamase (ESBL)- and AmpC β-lactamase (AmpC)-producing Escherichia coli from livestock and meat represent a zoonotic risk and biocontrol solutions are needed to prevent transmission to humans. Methods: In this study, we established a representative collection of animal-origin ESBL/AmpC E. coli as target to test the antimicrobial potential of bacteriophages. Results: Bioinformatic analysis of whole-genome sequence data of 198 ESBL/AmpC E. coli from pigs, broilers, and broiler meat identified strains belonging to all known E. coli phylogroups and 65 multilocus sequence types. Various ESBL/AmpC genes and plasmid types were detected with expected source-specific patterns. Plaque assay using 15 phages previously isolated using the E. coli reference collection demonstrated that Warwickvirus phages showed the broadest host range, killing up to 26 strains. Conclusions:154/198 strains were resistant to infection by all phages tested, suggesting a need for isolating phages specific for ESBL/AmpC E. coli. The strain collection described in this study is a useful resource fulfilling such need.
KW - bacteriophages
KW - biocontrol
KW - ESBL/AmpC-producing Escherichia coli
KW - extended-spectrum β-lactamase
KW - strain collection
U2 - 10.1089/phage.2023.0002
DO - 10.1089/phage.2023.0002
M3 - Journal article
C2 - 37214655
AN - SCOPUS:85151546146
VL - 4
SP - 35
EP - 45
JO - PHAGE: Therapy, Applications, and Research
JF - PHAGE: Therapy, Applications, and Research
SN - 2641-6530
IS - 1
ER -
ID: 343340376