A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae

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Standard

A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae. / Chávez, Sebastián; Beilharz, Traude; Rondón, Ana G.; Erdjument-Bromage, Hediye; Tempst, Paul; Svejstrup, Jesper Q.; Lithgow, Trevor; Aguilera, Andrés.

I: EMBO Journal, Bind 19, Nr. 21, 01.11.2000, s. 5824-5834.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Chávez, S, Beilharz, T, Rondón, AG, Erdjument-Bromage, H, Tempst, P, Svejstrup, JQ, Lithgow, T & Aguilera, A 2000, 'A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae', EMBO Journal, bind 19, nr. 21, s. 5824-5834. https://doi.org/10.1093/emboj/19.21.5824

APA

Chávez, S., Beilharz, T., Rondón, A. G., Erdjument-Bromage, H., Tempst, P., Svejstrup, J. Q., Lithgow, T., & Aguilera, A. (2000). A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae. EMBO Journal, 19(21), 5824-5834. https://doi.org/10.1093/emboj/19.21.5824

Vancouver

Chávez S, Beilharz T, Rondón AG, Erdjument-Bromage H, Tempst P, Svejstrup JQ o.a. A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae. EMBO Journal. 2000 nov. 1;19(21):5824-5834. https://doi.org/10.1093/emboj/19.21.5824

Author

Chávez, Sebastián ; Beilharz, Traude ; Rondón, Ana G. ; Erdjument-Bromage, Hediye ; Tempst, Paul ; Svejstrup, Jesper Q. ; Lithgow, Trevor ; Aguilera, Andrés. / A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae. I: EMBO Journal. 2000 ; Bind 19, Nr. 21. s. 5824-5834.

Bibtex

@article{8e3503f56dc544a2a8a508672d42393f,
title = "A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae",
abstract = "Transcription-induced recombination has been reported in all organisms from bacteria to mammals. We have shown previously that the yeast genes HPR1 and THO2 may be keys to the understanding of transcription-associated recombination, as they both affect transcription elongation and hyper-recombination in a concerted manner. Using a yeast strain that has the wild-type THO2 gene replaced by one encoding a His6-HA-tagged version, we have isolated an oligomeric complex containing four proteins: Tho2, Hpr1, Mft1 and a novel protein that we have named Thp2. We have reciprocally identified a complex containing Hpr1, Tho2 and Mft1 using anti-Mft1 antibodies in immunoprecipitation experiments. The protein complex is mainly nuclear; therefore, Tho2 and Hpr1 are physically associated. Like hpr1Δ and tho2Δ cells, mft1Δ and thp2Δ cells show mitotic hyper-recombination and impaired transcription elongation, in particular, through the bacterial lacZ sequence. Hyper-recombination conferred by mft1Δ and thp2Δ is only observed in DNA regions under transcription conditions. We propose that this protein complex acts as a functional unit connecting transcription elongation with the incidence of mitotic recombination.",
keywords = "HPR1, MFT1, Mitotic recombination, THO protein complex, THO2, THP2, Transcription elongation, Transcription-associated recombination",
author = "Sebasti{\'a}n Ch{\'a}vez and Traude Beilharz and Rond{\'o}n, {Ana G.} and Hediye Erdjument-Bromage and Paul Tempst and Svejstrup, {Jesper Q.} and Trevor Lithgow and Andr{\'e}s Aguilera",
year = "2000",
month = nov,
day = "1",
doi = "10.1093/emboj/19.21.5824",
language = "English",
volume = "19",
pages = "5824--5834",
journal = "E M B O Journal",
issn = "0261-4189",
publisher = "Wiley-Blackwell",
number = "21",

}

RIS

TY - JOUR

T1 - A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae

AU - Chávez, Sebastián

AU - Beilharz, Traude

AU - Rondón, Ana G.

AU - Erdjument-Bromage, Hediye

AU - Tempst, Paul

AU - Svejstrup, Jesper Q.

AU - Lithgow, Trevor

AU - Aguilera, Andrés

PY - 2000/11/1

Y1 - 2000/11/1

N2 - Transcription-induced recombination has been reported in all organisms from bacteria to mammals. We have shown previously that the yeast genes HPR1 and THO2 may be keys to the understanding of transcription-associated recombination, as they both affect transcription elongation and hyper-recombination in a concerted manner. Using a yeast strain that has the wild-type THO2 gene replaced by one encoding a His6-HA-tagged version, we have isolated an oligomeric complex containing four proteins: Tho2, Hpr1, Mft1 and a novel protein that we have named Thp2. We have reciprocally identified a complex containing Hpr1, Tho2 and Mft1 using anti-Mft1 antibodies in immunoprecipitation experiments. The protein complex is mainly nuclear; therefore, Tho2 and Hpr1 are physically associated. Like hpr1Δ and tho2Δ cells, mft1Δ and thp2Δ cells show mitotic hyper-recombination and impaired transcription elongation, in particular, through the bacterial lacZ sequence. Hyper-recombination conferred by mft1Δ and thp2Δ is only observed in DNA regions under transcription conditions. We propose that this protein complex acts as a functional unit connecting transcription elongation with the incidence of mitotic recombination.

AB - Transcription-induced recombination has been reported in all organisms from bacteria to mammals. We have shown previously that the yeast genes HPR1 and THO2 may be keys to the understanding of transcription-associated recombination, as they both affect transcription elongation and hyper-recombination in a concerted manner. Using a yeast strain that has the wild-type THO2 gene replaced by one encoding a His6-HA-tagged version, we have isolated an oligomeric complex containing four proteins: Tho2, Hpr1, Mft1 and a novel protein that we have named Thp2. We have reciprocally identified a complex containing Hpr1, Tho2 and Mft1 using anti-Mft1 antibodies in immunoprecipitation experiments. The protein complex is mainly nuclear; therefore, Tho2 and Hpr1 are physically associated. Like hpr1Δ and tho2Δ cells, mft1Δ and thp2Δ cells show mitotic hyper-recombination and impaired transcription elongation, in particular, through the bacterial lacZ sequence. Hyper-recombination conferred by mft1Δ and thp2Δ is only observed in DNA regions under transcription conditions. We propose that this protein complex acts as a functional unit connecting transcription elongation with the incidence of mitotic recombination.

KW - HPR1

KW - MFT1

KW - Mitotic recombination

KW - THO protein complex

KW - THO2

KW - THP2

KW - Transcription elongation

KW - Transcription-associated recombination

UR - http://www.scopus.com/inward/record.url?scp=0034329461&partnerID=8YFLogxK

U2 - 10.1093/emboj/19.21.5824

DO - 10.1093/emboj/19.21.5824

M3 - Journal article

C2 - 11060033

AN - SCOPUS:0034329461

VL - 19

SP - 5824

EP - 5834

JO - E M B O Journal

JF - E M B O Journal

SN - 0261-4189

IS - 21

ER -

ID: 331575221