A porcine brain-wide RNA editing landscape
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A porcine brain-wide RNA editing landscape. / Huang, Jinrong; Lin, Lin; Dong, Zhanying; Yang, Ling; Zheng, Tianyu; Gu, Weiwang; Zhang, Yan; Yin, Tailang; Sjöstedt, Evelina; Mulder, Jan; Uhlén, Mathias; Kristiansen, Karsten; Bolund, Lars; Luo, Yonglun.
I: Communications Biology , Bind 4, 717, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - A porcine brain-wide RNA editing landscape
AU - Huang, Jinrong
AU - Lin, Lin
AU - Dong, Zhanying
AU - Yang, Ling
AU - Zheng, Tianyu
AU - Gu, Weiwang
AU - Zhang, Yan
AU - Yin, Tailang
AU - Sjöstedt, Evelina
AU - Mulder, Jan
AU - Uhlén, Mathias
AU - Kristiansen, Karsten
AU - Bolund, Lars
AU - Luo, Yonglun
PY - 2021
Y1 - 2021
N2 - Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is an essential post-transcriptional modification. Although hundreds of thousands of RNA editing sites have been reported in mammals, brain-wide analysis of the RNA editing in the mammalian brain remains rare. Here, a genome-wide RNA-editing investigation is performed in 119 samples, representing 30 anatomically defined subregions in the pig brain. We identify a total of 682,037 A-to-I RNA editing sites of which 97% are not identified before. Within the pig brain, cerebellum and olfactory bulb are regions with most edited transcripts. The editing level of sites residing in protein-coding regions are similar across brain regions, whereas region-distinct editing is observed in repetitive sequences. Highly edited conserved recoding events in pig and human brain are found in neurotransmitter receptors, demonstrating the evolutionary importance of RNA editing in neurotransmission functions. Although potential data biases caused by age, sex or health status are not considered, this study provides a rich resource to better understand the evolutionary importance of post-transcriptional RNA editing. Huang et al performed a genome-wide RNA editing investigation in the porcine brain in which they found over 680,000 A-to-I RNA editing sites. They identified conserved recoding events between pig and human brains thus providing an extensive resource to aid our understanding of the evolutionary importance of post-transcriptional RNA editing.
AB - Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is an essential post-transcriptional modification. Although hundreds of thousands of RNA editing sites have been reported in mammals, brain-wide analysis of the RNA editing in the mammalian brain remains rare. Here, a genome-wide RNA-editing investigation is performed in 119 samples, representing 30 anatomically defined subregions in the pig brain. We identify a total of 682,037 A-to-I RNA editing sites of which 97% are not identified before. Within the pig brain, cerebellum and olfactory bulb are regions with most edited transcripts. The editing level of sites residing in protein-coding regions are similar across brain regions, whereas region-distinct editing is observed in repetitive sequences. Highly edited conserved recoding events in pig and human brain are found in neurotransmitter receptors, demonstrating the evolutionary importance of RNA editing in neurotransmission functions. Although potential data biases caused by age, sex or health status are not considered, this study provides a rich resource to better understand the evolutionary importance of post-transcriptional RNA editing. Huang et al performed a genome-wide RNA editing investigation in the porcine brain in which they found over 680,000 A-to-I RNA editing sites. They identified conserved recoding events between pig and human brains thus providing an extensive resource to aid our understanding of the evolutionary importance of post-transcriptional RNA editing.
KW - ADENOSINE
KW - ALIGNMENT
KW - TRAFFICKING
KW - EXPRESSION
KW - DEFICIENT
KW - DYNAMICS
KW - CHANNELS
KW - TARGETS
KW - BINDING
KW - MOUSE
U2 - 10.1038/s42003-021-02238-3
DO - 10.1038/s42003-021-02238-3
M3 - Journal article
C2 - 34112917
VL - 4
JO - Communications Biology
JF - Communications Biology
SN - 2399-3642
M1 - 717
ER -
ID: 273369898