A novel transcription factor UvCGBP1 regulates development and virulence of rice false smut fungus Ustilaginoidea virens
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A novel transcription factor UvCGBP1 regulates development and virulence of rice false smut fungus Ustilaginoidea virens. / Chen, Xiaoyang; Li, Pingping; Liu, Hao; Chen, Xiaolin; Huang, Junbin; Luo, Chaoxi; Li, Guotian; Hsiang, Tom; Collinge, David B.; Zheng, Lu.
I: Virulence, Bind 12, Nr. 1, 2021, s. 1563-1579.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - A novel transcription factor UvCGBP1 regulates development and virulence of rice false smut fungus Ustilaginoidea virens
AU - Chen, Xiaoyang
AU - Li, Pingping
AU - Liu, Hao
AU - Chen, Xiaolin
AU - Huang, Junbin
AU - Luo, Chaoxi
AU - Li, Guotian
AU - Hsiang, Tom
AU - Collinge, David B.
AU - Zheng, Lu
PY - 2021
Y1 - 2021
N2 - Ustilaginoidea virens, causing rice false smut (RFS) is an economically important ascomycetous fungal pathogen distributed in rice-growing regions worldwide. Here, we identified a novel transcription factor UvCGBP1 (Cutinase G-box binding protein) from this fungus, which is unique to ascomycetes. Deletion of UvCGBP1 affected development and virulence of U. virens. A total of 865 downstream target genes of UvCGBP1 was identified using ChIP-seq and the most significant KEGG enriched functional pathway was the MAPK signaling pathway. Approximately 36% of target genes contain the AGGGG (G-box) motif in their promoter. Among the targets, deletion of UvCGBP1 affected transcriptional and translational levels of UvPmk1 and UvSlt2, both of which were important in virulence. ChIP-qPCR, yeast one-hybrid and EMSA confirmed that UvCGBP1 can bind the promoter of UvPmk1 or UvSlt2. Overexpression of UvPmk1 in the ∆UvCGBP1-33 mutant restored partially its virulence and hyphae growth, indicating that UvCGBP1 could function via the MAPK pathway to regulate fungal virulence. Taken together, this study uncovered a novel regulatory mechanism of fungal virulence linking the MAPK pathway mediated by a G-box binding transcription factor, UvCGBP1.
AB - Ustilaginoidea virens, causing rice false smut (RFS) is an economically important ascomycetous fungal pathogen distributed in rice-growing regions worldwide. Here, we identified a novel transcription factor UvCGBP1 (Cutinase G-box binding protein) from this fungus, which is unique to ascomycetes. Deletion of UvCGBP1 affected development and virulence of U. virens. A total of 865 downstream target genes of UvCGBP1 was identified using ChIP-seq and the most significant KEGG enriched functional pathway was the MAPK signaling pathway. Approximately 36% of target genes contain the AGGGG (G-box) motif in their promoter. Among the targets, deletion of UvCGBP1 affected transcriptional and translational levels of UvPmk1 and UvSlt2, both of which were important in virulence. ChIP-qPCR, yeast one-hybrid and EMSA confirmed that UvCGBP1 can bind the promoter of UvPmk1 or UvSlt2. Overexpression of UvPmk1 in the ∆UvCGBP1-33 mutant restored partially its virulence and hyphae growth, indicating that UvCGBP1 could function via the MAPK pathway to regulate fungal virulence. Taken together, this study uncovered a novel regulatory mechanism of fungal virulence linking the MAPK pathway mediated by a G-box binding transcription factor, UvCGBP1.
KW - ChIP-seq
KW - MAPK pathway
KW - Ustilaginoidea virens
KW - UvCGBP1
KW - virulence
U2 - 10.1080/21505594.2021.1936768
DO - 10.1080/21505594.2021.1936768
M3 - Journal article
C2 - 34348597
AN - SCOPUS:85112022938
VL - 12
SP - 1563
EP - 1579
JO - Virulence
JF - Virulence
SN - 2150-5594
IS - 1
ER -
ID: 276161461