A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method
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A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method. / Könnings, Stephanie; Copland, Melissa J; Davies, Nigel M; Rades, Thomas.
I: International Journal of Pharmaceutics, Bind 241, Nr. 2, 25.07.2002, s. 385-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method
AU - Könnings, Stephanie
AU - Copland, Melissa J
AU - Davies, Nigel M
AU - Rades, Thomas
PY - 2002/7/25
Y1 - 2002/7/25
N2 - This study describes the development of a method for the incorporation of fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes (ISCOMs) prepared by the hydration method. Conjugation of palmitic acid was performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA, with subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both pFITC-OVA and FITC-pOVA, but not FITC-OVA, could be incorporated into ISCOMs and other non-liposomal colloidal structures. The degree of incorporation of pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if ISCOMs are the predominant colloids in the system.
AB - This study describes the development of a method for the incorporation of fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes (ISCOMs) prepared by the hydration method. Conjugation of palmitic acid was performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA, with subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both pFITC-OVA and FITC-pOVA, but not FITC-OVA, could be incorporated into ISCOMs and other non-liposomal colloidal structures. The degree of incorporation of pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if ISCOMs are the predominant colloids in the system.
KW - Adjuvants, Immunologic
KW - Drug Delivery Systems
KW - Fluorescein-5-isothiocyanate
KW - Fluorescent Dyes
KW - Ovalbumin
KW - Saponins
M3 - Journal article
C2 - 12100866
VL - 241
SP - 385
EP - 389
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
IS - 2
ER -
ID: 46408585