A gain-of-function suppressor screen for genes involved in dorsal-ventral boundary formation in the Drosophila wing
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
A gain-of-function suppressor screen for genes involved in dorsal-ventral boundary formation in the Drosophila wing. / Bejarano, Fernando; Luque, Carlos M; Herranz, Héctor; Rafel, Neus; Pham, Thu Thuy; Milán, Marco.
I: Genetics, Bind 178, Nr. 1, 01.2008, s. 307-23.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - A gain-of-function suppressor screen for genes involved in dorsal-ventral boundary formation in the Drosophila wing
AU - Bejarano, Fernando
AU - Luque, Carlos M
AU - Herranz, Héctor
AU - Rafel, Neus
AU - Pham, Thu Thuy
AU - Milán, Marco
PY - 2008/1
Y1 - 2008/1
N2 - The Drosophila wing primordium is subdivided into a dorsal (D) and a ventral (V) compartment by the activity of the LIM-homeodomain protein Apterous in D cells. Cell interactions between D and V cells induce the activation of Notch at the DV boundary. Notch is required for the maintenance of the compartment boundary and the growth of the wing primordium. Beadex, a gain-of-function allele of dLMO, results in increased levels of dLMO protein, which interferes with the activity of Apterous and results in defects in DV axis formation. We performed a gain-of-function enhancer-promoter (EP) screen to search for suppressors of Beadex when overexpressed in D cells. We identified 53 lines corresponding to 35 genes. Loci encoding for micro-RNAs and proteins involved in chromatin organization, transcriptional control, and vesicle trafficking were characterized in the context of dLMO activity and DV boundary formation. Our results indicate that a gain-of-function genetic screen in a sensitized background, as opposed to classical loss-of-function-based screenings, is a very efficient way to identify redundant genes involved in a developmental process.
AB - The Drosophila wing primordium is subdivided into a dorsal (D) and a ventral (V) compartment by the activity of the LIM-homeodomain protein Apterous in D cells. Cell interactions between D and V cells induce the activation of Notch at the DV boundary. Notch is required for the maintenance of the compartment boundary and the growth of the wing primordium. Beadex, a gain-of-function allele of dLMO, results in increased levels of dLMO protein, which interferes with the activity of Apterous and results in defects in DV axis formation. We performed a gain-of-function enhancer-promoter (EP) screen to search for suppressors of Beadex when overexpressed in D cells. We identified 53 lines corresponding to 35 genes. Loci encoding for micro-RNAs and proteins involved in chromatin organization, transcriptional control, and vesicle trafficking were characterized in the context of dLMO activity and DV boundary formation. Our results indicate that a gain-of-function genetic screen in a sensitized background, as opposed to classical loss-of-function-based screenings, is a very efficient way to identify redundant genes involved in a developmental process.
KW - Animals
KW - Biological Transport
KW - Body Patterning
KW - Chromatin
KW - Drosophila Proteins
KW - Drosophila melanogaster
KW - Genes, Insect
KW - Genes, Suppressor
KW - In Situ Hybridization
KW - Membrane Fusion
KW - Phenotype
KW - Phosphorylation
KW - Protein Structure, Tertiary
KW - Receptors, Notch
KW - Sequence Homology, Amino Acid
KW - Suppression, Genetic
KW - Transcription, Genetic
KW - Transport Vesicles
KW - Wing
U2 - 10.1534/genetics.107.081869
DO - 10.1534/genetics.107.081869
M3 - Journal article
C2 - 18202376
VL - 178
SP - 307
EP - 323
JO - Genetics
JF - Genetics
SN - 1943-2631
IS - 1
ER -
ID: 119179626